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Author(s): Bárbara M. Oliveira
Alexandra Correia
Ana Pinto
Pedro Meireles
Paula G Ferreira
Manuel Vilanova
Luzia Teixeira
Title: T cells in mesenteric and subcutaneous adipose tissue of Holstein-Friesian cows
Issue Date: 2019-03-04
Abstract: The importance of immune cells present in the adipose tissue to metabolic homeostasis has been increasingly recognized. Nevertheless, in bovines few studies have so far addressed the immune cell populations resident in this tissue. Here we developed an eight-colour flow cytometry panel to address T cell populations present in bovine adipose tissue. Our results showed that gamma dT cells, CD4(+) and CD8(+) CD3(+) non-gamma dT cells, as well as NK cells, are present in the mesenteric and subcutaneous adipose tissue of Holstein-Friesian cows. The frequency of both gamma dT cells and CD8(+) non-gamma dT cells was found higher in mesenteric than in subcutaneous adipose tissue. The majority of T cells in adipose tissue presented a CD45RO(+)CD62L(-) phenotype, characteristic of effector memory cells, and the frequency of these cellular populations was higher than in the blood. The ratio of CD4(+) T cells over CD8(+) T cells was similar between subcutaneous and mesenteric adipose tissue but different from the one found in blood. Overall, our results highlight particular phenotypic characteristics of bovine adipose tissue T cell populations.
Related Information: info:eu-repo/grantAgreement/Agência de Inovação, S.A./Programa Operacional Factores de Competitividade/224-PTDC/CVT-WEL/3079/2014/Estudo da resposta imunológica no tecido adiposo de hospedeiros infectados com Neospora Caninum/ADIPONEO
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:ICBAS - Artigo em Revista Científica Internacional

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