Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/115806
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dc.creatorAraujo, R
dc.creatorSantos, JMO
dc.creatorFernandes, M
dc.creatorDias, F
dc.creatorSousa, H
dc.creatorRibeiro, J
dc.creatorMargarida Bastos
dc.creatorOliveira, PA
dc.creatorCarmo, D
dc.creatorCasaca, F
dc.creatorSilva, S
dc.creatorMedeiros, R
dc.creatorGil da Costa, R. M.
dc.date.accessioned2019-02-07T17:01:40Z-
dc.date.available2019-02-07T17:01:40Z-
dc.date.issued2018
dc.identifier.issn0171-5216
dc.identifier.othersigarra:273083
dc.identifier.urihttps://repositorio-aberto.up.pt/handle/10216/115806-
dc.description.abstractPurpose Persistent human papillomavirus (HPV) infection is associated with the development of certain types of cancer and the dysregulation of microRNAs has been implicated in HPV-associated carcinogenesis. This is the case of microRNA-146a (miR-146a), which is thought to regulate tumor-associated inflammation. We sought to investigate the expression levels of miR-146a during HPV16-mediated carcinogenesis using skin samples from K14-HPV16 transgenic mice which develop the consecutive phases of the carcinogenesis process. Methods Female transgenic (HPV+/-) and wild-type (HPV-/-) mice were sacrificed at 24-26 weeks-old or 28-30 weeks-old. Chest and ear skin samples from HPV+/- and HPV-/- mice were histologically classified and used for microRNA extraction and quantification by qPCR. Results Chest skin samples from 24 to 26 weeks-old HPV+/- mice presented diffuse epidermal hyperplasia and only 22.5% showed multifocal dysplasia, while at 28-30 weeks-old all (100.0%) HPV+/- animals showed epidermal dysplasia. All HPV+/- ear skin samples showed carcinoma in situ (CIS). MiR-146a expression levels were higher in HPV+/- compared to HPV-/- mice (p = 0.006). There was also an increase in miR-146a expression in dysplastic skin lesions compared with hyperplasic lesions (p = 0.011). Samples showing CIS had a significant decrease in miR-146a expression when compared to samples showing epidermal hyperplasia (p = 0.018) and epidermal dysplasia (p = 0.009). Conclusions These results suggest that HPV16 induces the overexpression of miR-146a in the initial stages of carcinogenesis (hyperplasia and dysplasia), whereas decreases its expression at later stages (CIS). Taken together, these data implicate and suggest different roles of miR-146a in HPV-mediated carcinogenesis.
dc.language.isoeng
dc.relationinfo:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE
dc.rightsrestrictedAccess
dc.titleExpression profile of microRNA-146a along HPV-induced multistep carcinogenesis: a study in HPV16 transgenic mice
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoFaculdade de Engenharia
dc.identifier.doi10.1007/s00432-017-2549-5
dc.identifier.authenticusP-00N-8V7
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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