Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/114756
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dc.creatorRamos, A
dc.creatorCarvalho, T
dc.creatorRibeiro, M
dc.creatorGuimarães, JT
dc.date.accessioned2018-08-27T11:34:55Z-
dc.date.available2018-08-27T11:34:55Z-
dc.date.issued2016
dc.identifier.issn1027-3719 
dc.identifier.urihttp://hdl.handle.net/10216/114756-
dc.description.abstractSETTING: The ability to rapidly distinguish between Mycobacterium tuberculosis complex (MTC) and non-tuberculous mycobacteria (NTM) is critical in clinical practice. OBJECTIVE: To evaluate the usefulness of an immunochromatographic (IC) assay to distinguish between MTC and NTM. DESIGN: We analysed a panel of 145 cultures from 128 patients. The routine molecular identification approaches, such as the AccuProbe™ Mycobacterium tuberculosis complex culture identification test and GenoType(®) Mycobacterium assays, were used as reference methods. RESULTS: Of the 101 positive cultures, 98 were correctly identified using the Capilia™ TB-Neo Assay. Of the three discordant isolates, one was identified as M. bovis bacille Calmette-Guérin (BCG) and two as M. tuberculosis. Although we have not performed the sequencing of these strains, some false-negative results have been described due to mutations in the mpb64 gene or with some M. bovis BCG strains. We did not observe false-positive results or any cross-reaction with 22 NTM strains, 12 non-mycobacterial micro-organisms and 10 negative cultures. CONCLUSION: We report good overall performance (sensitivity 97%, specificity 100%, positive predictive value 100% and negative predictive value 96%) of this rapid assay that is easy to perform and interpret and does not require sample preparation, trained technicians or expensive equipment.
dc.language.isoeng
dc.relation.ispartofInt J Tuberc Lung Dis, vol. 30(6), p. 1183-1190
dc.rightsopenAccess
dc.subjectTuberculosis
dc.subjectTuberculous mycobacteria
dc.subjectNon-tuberculous mycobacteria
dc.titleCapilia™ TB-Neo assay: a new tool for rapid distinction between tuberculous and non-tuberculous mycobacteria
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoInstituto de Saúde Pública
dc.identifier.doi10.5588/ijtld.15.0528
dc.relation.publisherversionhttp://www.ingentaconnect.com/content/iuatld/ijtld/2016/00000020/00000006/art00008;jsessionid=369lm7q5hke8j.x-ic-live-01
Appears in Collections:ISPUP - Artigo em Revista Científica Internacional

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