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https://hdl.handle.net/10216/114739
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DC Field | Value | Language |
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dc.creator | da Silva, DM | |
dc.creator | Vieira, TM | |
dc.creator | Pereira, AM | |
dc.creator | Moreira, A | |
dc.creator | Delgado, JL | |
dc.date.accessioned | 2018-08-27T11:34:54Z | - |
dc.date.available | 2018-08-27T11:34:54Z | - |
dc.date.issued | 2016 | |
dc.identifier.issn | 2045-7022 | |
dc.identifier.uri | http://hdl.handle.net/10216/114739 | - |
dc.description.abstract | Background: Challenge tests for food-dependent exercise-induced anaphylaxis (FDEIA) carry some risk and have a high rate of false negatives. Our aim was to explore the usefulness of an in vitro immunodepletion assay and an allergen microarray test in the identification of IgE-mediated cross-reactive food allergens in patients with suspected FDEIA or food-dependent exercise-induced urticaria and panallergen sensitization. Methods: Three patients with a history of food dependent exercise induced urticaria/anaphylaxis and food panallergen sensitization in whom a food-exercise challenge was not feasible were selected: a 25-year-old man with cholinergic urticaria who experienced generalized urticaria and angioedema during a soccer match after drinking a peach-based soft drink; a 19-year-old woman with allergic rhinitis and controlled asthma who experienced anaphylactic shock while playing soccer, having eaten walnuts in the previous 90 min; and a 57-year-old man with baker’s asthma who experienced four episodes of anaphylaxis during exercise after ingesting wheat-containing food. All individuals underwent a diagnostic work-up with skin prick tests, specific IgE (sIgE) and ImmunoCAP ISAC test. For the in vitro immunodepletion procedure, patients’ serum was pre-incubated with the suspected native allergen (peach, walnut, or wheat) in solid phase (ImmunoCAP). The eluted serum, containing unbound IgE, was collected and samples were re-tested using Immunocap ISAC 112 and compared with baseline results. Results: All individuals were sensitized to lipid transfer proteins. The first patient was sensitized to Pru p 3, Cor a 8, Jug r 3, and Ara h 9; after pre-incubation with peach there was 100% depletion of sIgE to all components. The second patient was sensitized to Pru p 3, Cor a 8, Jug r 3, and Ara h 9; immunodepletion with walnut depleted sIgE to Ara h 9 by 67%, Pru p 3 and Pla a 3 (60%), Art v 3 (75%), Jug r 3 (88%), and Cor a 8 (100%). The third patient was sensitized to Pru p 3, Jug r 3, Ara h 9, and Tri a 14; immunodepletion with wheat depleted Tri a 14 only (100%). Conclusions: In vitro immunodepletion might be a useful diagnostic tool in food dependent exercise induced urticaria/anaphylaxis with panallergen sensitization, particularly for identifying the culprit allergen and guiding dietary elimination recommendations. | |
dc.language.iso | eng | |
dc.relation.ispartof | Clin Transl Allergy, vol. 6, p. 46 | |
dc.rights | openAccess | |
dc.subject | Food-Dependent Exercise-Induced Anaphylaxis | |
dc.subject | FDEIA | |
dc.title | Cross-reactive LTP sensitization in food-dependent exercise-induced urticaria/anaphylaxis: a pilot study of a component-resolved and in vitro depletion approach | |
dc.type | Artigo em Revista Científica Internacional | |
dc.contributor.uporto | Instituto de Saúde Pública | |
dc.identifier.doi | 10.1186/s13601-016-0136-5 | |
dc.relation.publisherversion | https://ctajournal.biomedcentral.com/articles/10.1186/s13601-016-0136-5 | |
Appears in Collections: | ISPUP - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
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SilvaD2016.pdf | 2.05 MB | Adobe PDF | View/Open |
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