Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/114508
Author(s): | Maia, AF Tanenbaum, ME Galli, M Lelieveld, D Egan, DA Gassmann, R Sunkel, CE den, Heuvel, S Medema, RH |
Title: | Genome-wide RNAi screen for synthetic lethal interactions with the C. elegans kinesin-5 homolog BMK-1 |
Publisher: | Nature Publishing Group |
Issue Date: | 2015 |
Abstract: | Kinesins are a superfamily of microtubule-based molecular motors that perform various transport needs and have essential roles in cell division. Among these, the kinesin-5 family has been shown to play a major role in the formation and maintenance of the bipolar mitotic spindle. Moreover, recent work suggests that kinesin-5 motors may have additional roles. In contrast to most model organisms, the sole kinesin-5 gene in Caenorhabditis elegans, bmk-1, is not required for successful mitosis and animals lacking bmk-1 are viable and fertile. To gain insight into factors that may act redundantly with BMK-1 in spindle assembly and to identify possible additional cellular pathways involving BMK-1, we performed a synthetic lethal screen using the bmk-1 deletion allele ok391. We successfully knocked down 82% of the C. elegans genome using RNAi and assayed viability in bmk-1(ok391) and wild type strains using an automated high-throughput approach based on fluorescence microscopy. The dataset includes a final list of 37 synthetic lethal interactions whose further study is likely to provide insight into kinesin-5 function. |
Subject: | Animals Caenorhabditis elegans Caenorhabditis elegans Proteins/genetics Caenorhabditis elegans Proteins/metabolism Genes, Lethal Genome, Helminth Kinesin/genetics Kinesin/metabolism Microtubule-Associated Proteins/genetics Microtubule-Associated Proteins/metabolism RNA Interference Signal Transduction Spindle Apparatus |
URI: | http://hdl.handle.net/10216/114508 |
Source: | Scientific data, 2:150020 |
Related Information: | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F71364%2F2010/PT info:eu-repo/grantAgreement/FCT/COMPETE/120366/PT |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | openAccess |
License: | https://creativecommons.org/licenses/by/4.0/ |
Appears in Collections: | I3S - Artigo em Revista Científica Internacional |
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Maia A et al_2015.pdf | 989.39 kB | Adobe PDF | View/Open |
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