Please use this identifier to cite or link to this item: http://hdl.handle.net/10216/110923
Author(s): Silva, AM
Osório, DS
Pereira, AJ
Maiato, H
Pinto, IM
Rubinstein, B
Gassmann, R
Telley, IA
Carvalho, AX
Title: Robust gap repair in the contractile ring ensures timely completion of cytokinesis.
Publisher: Rockefeller University Press
Issue Date: 2016
Abstract: Cytokinesis in animal cells requires the constriction of an actomyosin contractile ring, whose architecture and mechanism remain poorly understood. We use laser microsurgery to explore the biophysical properties of constricting rings in Caenorhabditis elegans embryos. Laser cutting causes rings to snap open. However, instead of disintegrating, ring topology recovers and constriction proceeds. In response to severing, a finite gap forms and is repaired by recruitment of new material in an actin polymerization-dependent manner. An open ring is able to constrict, and rings repair from successive cuts. After gap repair, an increase in constriction velocity allows cytokinesis to complete at the same time as controls. Our analysis demonstrates that tension in the ring increases while net cortical tension at the site of ingression decreases throughout constriction and suggests that cytokinesis is accomplished by contractile modules that assemble and contract autonomously, enabling local repair of the actomyosin network. Consequently, cytokinesis is a highly robust process impervious to discontinuities in contractile ring structure.
Subject: Actomyosin/metabolism
Animals
Bridged Bicyclo Compounds, Heterocyclic/pharmacology
Caenorhabditis elegans/cytology
Caenorhabditis elegans/drug effects
Caenorhabditis elegans/embryology
Cytokinesis
Embryo, Nonmammalian/cytology
Lasers
Microsurgery
Thiazolidines/pharmacology
URI: http://hdl.handle.net/10216/110923
Series: The Journal of cell biology, vol. 215(6), p. 789-799
Related Information: info:eu-repo/grantAgreement/FCT/5876-PPCDTI/125211/PT
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F95707%2F2013/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
License: https://creativecommons.org/licenses/by-nc-sa/4.0/
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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