Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/110358
Author(s): Costa, FM
Maia, SR
Gomes, PA
Martins, MC
Title: Dhvar5 antimicrobial peptide (AMP) chemoselective covalent immobilization results on higher antiadherence effect than simple physical adsorption
Publisher: Elsevier
Issue Date: 2015
Abstract: Bacterial colonization and subsequent biofilm formation is still one of the major problems associated with medical devices. Antimicrobial peptides (AMP) immobilization onto biomaterials surface is a promising strategy to avoid bacterial colonization. However, a correct peptide orientation and exposure from the surface is essential to maintain AMP antimicrobial activity. This work aims to evaluate the effect of the immobilization on antibacterial activity of Dhvar5 (LLLFLLKKRKKRKY), an AMP with a head-to-tail amphipathicity. Dhvar5 was linked to thin chitosan coatings in i) a controlled orientation and exposure, testing covalent immobilization of its N- or C-terminus and using spacers with different lengths and flexibilities or in ii) a random orientation by physical adsorption. Chitosan coating was chosen due to its antimicrobial properties and readiness to be functionalized. Surface characterization demonstrated the chemoselective immobilization of the peptide with different spacers in a similar concentration (∼2 ng/cm2). Efficacy assays demonstrated that covalent immobilization of Dhvar5 exposing its cationic end, improves the chitosan coating antimicrobial effect by decreasing Methicillin-resistant Staphylococcus aureus (MRSA) colonization. This effect was enhanced when longer spacers were used independently of their flexibility. In opposite, immobilized Dhvar5 exposing its hydrophobic end has no effect on bacterial adhesion to chitosan, and when adsorbed in a random orientation even induces bacterial adhesion to chitosan coating.
Subject: Adsorption
Anti-Infective Agents/chemistry
Antimicrobial Cationic Peptides/chemistry
Bacteria/drug effects
Bacterial Adhesion
Bacterial Proteins/chemistry
Chitosan/chemistry
Coated Materials Biocompatible/chemistry
Histatins/chemistry
Methicillin-Resistant Staphylococcus aureus/drug effects
Microbial Sensitivity Tests
Protein Structure, Tertiary
Spectroscopy Fourier Transform Infrared
Surface Properties
Water
URI: http://hdl.handle.net/10216/110358
Source: Biomaterials, vol. 52 p. 531-538
Related Information: info:eu-repo/grantAgreement/FCT/5876-PPCDTI/101484/PT
info:eu-repo/grantAgreement/FCT/COMPETE/132934/PT
info:eu-repo/grantAgreement/FCT/COMPETE/132952/PT
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F72471%2F2010/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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