Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/110354
Author(s): Teixeira, F
Castro, H
Cruz, T
Tse, E
Koldewey, P
Southworth, DR
Tomás, AM
Jakob, U
Title: Mitochondrial peroxiredoxin functions as crucial chaperone reservoir in Leishmania infantum
Publisher: National Academy of Sciences
Issue Date: 2015
Abstract: Cytosolic eukaryotic 2-Cys-peroxiredoxins have been widely reported to act as dual-function proteins, either detoxifying reactive oxygen species or acting as chaperones to prevent protein aggregation. Several stimuli, including peroxide-mediated sulfinic acid formation at the active site cysteine, have been proposed to trigger the chaperone activity. However, the mechanism underlying this activation and the extent to which the chaperone function is crucial under physiological conditions in vivo remained unknown. Here we demonstrate that in the vector-borne protozoan parasite Leishmania infantum, mitochondrial peroxiredoxin (Prx) exerts intrinsic ATP-independent chaperone activity, protecting a wide variety of different proteins against heat stress-mediated unfolding in vitro and in vivo. Activation of the chaperone function appears to be induced by temperature-mediated restructuring of the reduced decamers, promoting binding of unfolding client proteins in the center of Prx's ringlike structure. Client proteins are maintained in a folding-competent conformation until restoration of nonstress conditions, upon which they are released and transferred to ATP-dependent chaperones for refolding. Interference with client binding impairs parasite infectivity, providing compelling evidence for the in vivo importance of Prx's chaperone function. Our results suggest that reduced Prx provides a mitochondrial chaperone reservoir, which allows L. infantum to deal successfully with protein unfolding conditions during the transition from insect to the mammalian hosts and to generate viable parasites capable of perpetuating infection.
Subject: Animals
Leishmania infantum/enzymology
Leishmania infantum/pathogenicity
Luciferases/metabolism
Molecular Chaperones/metabolism
Peroxiredoxins/metabolism
Protein Folding
Virulence
URI: http://hdl.handle.net/10216/110354
Source: Proc Natl Acad Sci U S A. 2015 Feb 17;112(7):E616-24
Related Information: info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F70438%2F2010/PT
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F80836%2F2011/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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