Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/110353
Author(s): Barisic, M
Sousa, RS
Tripathy, SK
Magiera, MM
Zaytsev, AV
Pereira, AL
Janke, C
Grishchuk, EL
Maiato, H
Title: Microtubule detyrosination guides chromosomes during mitosis
Publisher: American Association for the Advancement of Science
Issue Date: 2015
Abstract: Before chromosomes segregate into daughter cells, they align at the mitotic spindle equator, a process known as chromosome congression. Centromere-associated protein E (CENP-E)/Kinesin-7 is a microtubule plus-end-directed kinetochore motor required for congression of pole-proximal chromosomes. Because the plus-ends of many astral microtubules in the spindle point to the cell cortex, it remains unknown how CENP-E guides pole-proximal chromosomes specifically toward the equator. We found that congression of pole-proximal chromosomes depended on specific posttranslational detyrosination of spindle microtubules that point to the equator. In vitro reconstitution experiments demonstrated that CENP-E-dependent transport was strongly enhanced on detyrosinated microtubules. Blocking tubulin tyrosination in cells caused ubiquitous detyrosination of spindle microtubules, and CENP-E transported chromosomes away from spindle poles in random directions. Thus, CENP-E-driven chromosome congression is guided by microtubule detyrosination.
Subject: Bridged Bicyclo Compounds Heterocyclic/pharmacology
Cell Line, Tumor
Chromosomal Proteins
Non-Histone/antagonists & inhibitors
Chromosomal Proteins Non-Histone/genetics
Chromosomal Proteins Non-Histone/metabolism
Chromosome Segregation
Humans
Microtubules/metabolism
Mitosis
Molecular Imaging
Sarcosine/analogs & derivatives
Sarcosine/pharmacology
Spindle Apparatus/metabolism
Tubulin/metabolism
Tyrosine/metabolism
URI: http://hdl.handle.net/10216/110353
Source: Science, vol. 348(6236), p. 799-803.
Related Information: info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F66707%2F2009/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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