Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/110351
Author(s): Vasconcelos, DM
Gonçalves, RM
Almeida, CR
Pereira, IO
Oliveira, MI
Neves, N
Silva, AM
Ribeiro, AC
Cunha, C
Almeida, AR
Ribeiro, CC
Gil, AM
Seebach, E
Kynast, KL
Richter, W
Lamghari, M
Santos, SG
Barbosa, MA
Title: Fibrinogen scaffolds with immunomodulatory properties promote in vivo bone regeneration
Publisher: Elsevier
Issue Date: 2016
Abstract: The hypothesis behind this work is that fibrinogen (Fg), classically considered a pro-inflammatory protein, can promote bone repair/regeneration. Injury and biomaterial implantation naturally lead to an inflammatory response, which should be under control, but not necessarily minimized. Herein, porous scaffolds entirely constituted of Fg (Fg-3D) were implanted in a femoral rat bone defect and investigated at two important time points, addressing the bone regenerative process and the local and systemic immune responses, both crucial to elucidate the mechanisms of tissue remodelling. Fg-3D led to early infiltration of granulation tissue (6 days post-implantation), followed by bone defect closure, including periosteum repair (8 weeks post-injury). In the acute inflammatory phase (6 days) local gene expression analysis revealed significant increases of pro-inflammatory cytokines IL-6 and IL-8, when compared with non-operated animals. This correlated with modified proportions of systemic immune cell populations, namely increased T cells and decreased B, NK and NKT lymphocytes and myeloid cell, including the Mac-1+ (CD18+/CD11b+) subpopulation. At 8 weeks, Fg-3D led to decreased plasma levels of IL-1β and increased TGF-β1. Thus, our data supports the hypothesis, establishing a link between bone repair induced by Fg-3D and the immune response. In this sense, Fg-3D scaffolds may be considered immunomodulatory biomaterials.
Subject: Absorbable Implants
Animals
Bone Regeneration/drug effects
Bone Regeneration/immunology
Cytokines/immunology
Drug Implants/administration & dosage
Femoral Fractures/immunology
Femoral Fractures/pathology
Femoral Fractures/therapy
Fibrinogen/administration & dosage
Fibrinogen/chemistry
Fracture Healing/drug effects
Fracture Healing/immunology
Guided Tissue Regeneration/instrumentation
Immunologic Factors/administration & dosage
Male
Rats
Rats, Wistar
Tissue Scaffolds
Treatment Outcome
URI: http://hdl.handle.net/10216/110351
Source: Biomaterials, vol. 111, p. 163-178
Related Information: info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F85968%2F2012/PT
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F87516%2F2012/PT
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F37090%2F2007/PT
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F87071%2F2012/PT
info:eu-repo/grantAgreement/FCT/5876/147332/PT
Document Type: Artigo em Revista Científica Internacional
Rights: embargoedAccess
Embargo End Date: 2018-12-31
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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