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Author(s): Afonso, O
Matos, I
Pereira, AJ
Aguiar, P
Lampson, MA
Maiato, H
Title: Feedback control of chromosome separation by a midzone Aurora B gradient
Publisher: American Association for the Advancement of Science
Issue Date: 2014
Abstract: Accurate chromosome segregation during mitosis requires the physical separation of sister chromatids before nuclear envelope reassembly (NER). However, how these two processes are coordinated remains unknown. Here, we identified a conserved feedback control mechanism that delays chromosome decondensation and NER in response to incomplete chromosome separation during anaphase. A midzone-associated Aurora B gradient was found to monitor chromosome position along the division axis and to prevent premature chromosome decondensation by retaining Condensin I. PP1/PP2A phosphatases counteracted this gradient and promoted chromosome decondensation and NER. Thus, an Aurora B gradient appears to mediate a surveillance mechanism that prevents chromosome decondensation and NER until effective separation of sister chromatids is achieved. This allows the correction and reintegration of lagging chromosomes in the main nuclei before completion of NER.
Subject: Anaphase
Aurora Kinase B/antagonists & inhibitors
Aurora Kinase B/genetics
Aurora Kinase B/metabolism
Cell Line
Cell Line, Tumor
Chromosome Segregation/genetics
Chromosome Segregation/physiology
Feedback, Physiological
Nuclear Envelope/genetics
Nuclear Envelope/metabolism
Protein Phosphatase 1/metabolism
Protein Phosphatase 2/metabolism
Source: Science, vol. 345(6194), p. 332-336
Related Information: info:eu-repo/grantAgreement/FCT/5876-PPCDTI/112917/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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