Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/109584
Author(s): | Alonso, A Alves, C Suárez-Mier, MP Albarrán, C Pereira, L Fernandez de Simón, LF Martín, P Garcia, O Gusmão, L Sancho, M Amorim, A |
Title: | Mitochondrial DNA haplotyping revealed the presence of mixed up benign and neoplastic tissue sections from two individuals on the same prostatic biopsy slide |
Publisher: | BMJ Publishing Group |
Issue Date: | 2005 |
Abstract: | DNA typing was requested to investigate a presumptive cancer diagnosis error by confirming whether benign and cancerous prostatic tissue in the same presurgical haematoxylin and eosin stained slide belonged to the same person. After independent histological re-examination of the slide by a pathologist, manual slide dissection was used to guarantee independent and high recovery DNA isolation from each tissue section, avoiding carryover and background contamination. Nuclear DNA quantification performed by real time polymerase chain reaction (PCR) revealed the absence of human DNA for short tandem repeat (STR) typing. Mitochondrial DNA was only obtained by performing PCR of very short fragments ( approximately 100 bp), indicating high DNA degradation. Different low frequency hypervariable region I haplotypes were obtained from each tissue section (normal tissue section haplotype: 16224C, 16234T, 16311C, 16356C; cancer tissue section haplotype: 16256T, 16270T, 16293G). Only the normal tissue section haplotype matched that obtained from the patient's blood sample, indicating that the cancer tissue section originated from an unknown patient. These results supported the hypothesis of sample mix up during block processing or slide preparation by a carryover mechanism. Mitochondrial genetic typing is recommended to exclude the possibility of carryover artefacts when low DNA content and high degradation compromise conventional STR typing. |
Subject: | Artifacts Base Sequence Biopsy DNA Mitochondrial/analysis DNA Mitochondrial/genetics Haplotypes Humans Male Molecular Sequence Data Polymerase Chain Reaction/methods Prostatic Neoplasms/genetics Prostatic Neoplasms/pathology Sequence Analysis DNA/methods Tandem Repeat Sequences/genetics |
URI: | http://hdl.handle.net/10216/109584 |
Source: | Journal of Clinical Pathology, vol. 58(1), p. 83-6 |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | restrictedAccess |
Appears in Collections: | I3S - Artigo em Revista Científica Internacional |
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File | Description | Size | Format | |
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Alonso2005JCP.pdf Restricted Access | 320 kB | Adobe PDF | Request a copy |
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