Please use this identifier to cite or link to this item: http://hdl.handle.net/10216/108249
Author(s): Matos, C
Nóbrega, C
Louros, S
Almeida, B
Ferreiro, E
Valero, J
Almeida, L
Macedo-Ribeiro, S
Carvalho, A
Title: Ataxin-3 phosphorylation decreases neuronal defects in spinocerebellar ataxia type 3 models
Publisher: Rockefeller University Press
Issue Date: 2016
Abstract: Different neurodegenerative diseases are caused by aberrant elongation of repeated glutamine sequences normally found in particular human proteins. Although the proteins involved are ubiquitously distributed in human tissues, toxicity targets only defined neuronal populations. Changes caused by an expanded polyglutamine protein are possibly influenced by endogenous cellular mechanisms, which may be harnessed to produce neuroprotection. Here, we show that ataxin-3, the protein involved in spinocerebellar ataxia type 3, also known as Machado-Joseph disease, causes dendritic and synapse loss in cultured neurons when expanded. We report that S12 of ataxin-3 is phosphorylated in neurons and that mutating this residue so as to mimic a constitutive phosphorylated state counters the neuromorphologic defects observed. In rats stereotaxically injected with expanded ataxin-3–encoding lentiviral vectors, mutation of serine 12 reduces aggregation, neuronal loss, and synapse loss. Our results suggest that S12 plays a role in the pathogenic pathways mediated by polyglutamine-expanded ataxin-3 and that phosphorylation of this residue protects against toxicity.
Subject: Machado-joseph-disease
Protein ataxin-3
Polyglutamine disease
Mutant ataxin-3
Rat model
Huntingtin phosphorylation
Clinical-features
Expanded ataxin-3
Repeat expansion
Transgenic mice
URI: http://hdl.handle.net/10216/108249
Source: Journal of Cell Biology, vol. 212 (4), p. 465-480
Related Information: info:eu-repo/grantAgreement/FCT/SFRH/SFRH/BD/47160/2008/PT
info:eu-repo/grantAgreement/FCT/SFRH/SFRH/BPD/70783/2010/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
License: http://creativecommons.org/licenses/by-nc-sa/3.0/
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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