Please use this identifier to cite or link to this item: http://hdl.handle.net/10216/108242
Author(s): Alemi, M
Gaiteiro, C
Ribeiro, C
Santos, L
Gomes, J
Oliveira, S
Couraud, P
Weksler, B
Romero, I
Saraiva, MJ
Cardoso, I
Title: Transthyretin participates in beta-amyloid transport from the brain to the liver- involvement of the low-density lipoprotein receptor-related protein 1?
Publisher: Nature Publishing Group
Issue Date: 2016
Abstract: Transthyretin (TTR) binds Aβ peptide, preventing its deposition and toxicity. TTR is decreased in Alzheimer’s disease (AD) patients. Additionally, AD transgenic mice with only one copy of the TTR gene show increased brain and plasma Aβ levels when compared to AD mice with both copies of the gene, suggesting TTR involvement in brain Aβ efflux and/or peripheral clearance. Here we showed that TTR promotes Aβ internalization and efflux in a human cerebral microvascular endothelial cell line, hCMEC/D3. TTR also stimulated brain-to-blood but not blood-to-brain Aβ permeability in hCMEC/D3, suggesting that TTR interacts directly with Aβ at the blood-brain-barrier. We also observed that TTR crosses the monolayer of cells only in the brain-to-blood direction, as confirmed by in vivo studies, suggesting that TTR can transport Aβ from, but not into the brain. Furthermore, TTR increased Aβ internalization by SAHep cells and by primary hepatocytes from TTR+/+ mice when compared to TTR−/− animals. We propose that TTR-mediated Aβ clearance is through LRP1, as lower receptor expression was found in brains and livers of TTR−/− mice and in cells incubated without TTR. Our results suggest that TTR acts as a carrier of Aβ at the blood-brain-barrier and liver, using LRP1.
Subject: Growth-factor-i
Alzheimers-disease
Mouse model
A-beta
Cerebrospinal-fluid
Cholesterol-metabolism
Apolipoprotein-e
Clearance
Peptide
Binding
URI: http://hdl.handle.net/10216/108242
Source: Scientific Reports, vol. 6:20164
Related Information: info:eu-repo/grantAgreement/FCT/5876-PPCDTI/118076/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
License: http://creativecommons.org/licenses/by/4.0/
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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