Please use this identifier to cite or link to this item: http://hdl.handle.net/10216/108241
Author(s): Lourenço, T
Faria, J. Paes de
Bippes, C
Maia, J
Lopes-Da-Silva, J
Relvas, JB
Graõs, M
Title: Modulation of oligodendrocyte differentiation and maturation by combined biochemical and mechanical cues
Publisher: Nature Publishing Group
Issue Date: 2016
Abstract: Extracellular matrix (ECM) proteins play a key role during oligodendrogenesis. While fibronectin (FN) is involved in the maintenance and proliferation of oligodendrocyte progenitor cells (OPCs), merosin (MN) promotes differentiation into oligodendrocytes (OLs). Mechanical properties of the ECM also seem to affect OL differentiation, hence this study aimed to clarify the impact of combined biophysical and biochemical elements during oligodendrocyte differentiation and maturation using synthetic elastic polymeric ECM-like substrates. CG-4 cells presented OPC- or OL-like morphology in response to brain-compliant substrates functionalised with FN or MN, respectively. The expression of the differentiation and maturation markers myelin basic protein - MBP - and proteolipid protein - PLP - (respectively) by primary rat oligodendrocytes was enhanced in presence of MN, but only on brain-compliant conditions, considering the distribution (MBP) or amount (PLP) of the protein. It was also observed that maturation of OLs was attained earlier (by assessing PLP expression) by cells differentiated on MN-functionalised brain-compliant substrates than on standard culture conditions. Moreover, the combination of MN and substrate compliance enhanced the maturation and morphological complexity of OLs. Considering the distinct degrees of stiffness tested ranging within those of the central nervous system, our results indicate that 6.5 kPa is the most suitable rigidity for oligodendrocyte differentiation.
Subject: Atomic-force microscopy
Cell-differentiation
Myosin-ii
Morphological-differentiation
Extracellular-matrix
Multiple-sclerosis
Progenitor cells
Stem-cells
CNS
Specification
URI: http://hdl.handle.net/10216/108241
Source: Scientific Reports, vol. 6:21563
Related Information: info:eu-repo/grantAgreement/FCT/SFRH/SFRH/BPD/34834/2007/PT
Document Type: Artigo em Revista Científica Internacional
Rights: openAccess
License: http://creativecommons.org/licenses/by/4.0/
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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