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Author(s): Estela Lladosa
Sara C. Silvério
Oscar Rodríguez
José A. Teixeira
Eugénia A. Macedo
Title: Liquid-Liquid Equilibria of Polymer-Salt Aqueous Two-Phase Systems: UCON 50-HB-5100 with Potassium Citrate and (Sodium or Potassium) Formate at 23 C
Issue Date: 2012
Abstract: Aqueous two-phase systems (ATPS) are recognized as very suitable techniques for the recovery of targetsolutes in biological applications. Three new phase diagrams of (UCON 50-HB-5100 + potassiumcitrate + water), (UCON 50-HB-5100 + sodium formate + water), and (UCON 50-HB-5100 + potassium formate + water) systems were measured at 23 C. The binodal curves were successfully described using theempirical equation suggested by Merchuk and co-workers. The reliability of the tie-line data experimentally determined was evaluated using the equations reported by OthmerTobias and Bancroft and satisfactory linearity was obtained for all ATPS. Among the salts studied, potassium citrate proved to be themost effective in ATPS formation, providing the largest heterogeneous region. Besides, the effect of bothanions and cations in the size of the heterogeneous region and in the slope of the tie-lines has been compared. For the same salts and conditions, the heterogeneous region using UCON as the phase-formingpolymer is larger than using polyethylene glycol. Furthermore, laccase partition in the UCON-salt ATPSwas studied and it was found that in all cases enzyme partition occurred preferably to the bottom phase(salt-rich phase). Laccase concentration in the salt-rich phase was approximately 2-fold that in the topphase, thus UCON-salt ATPS can be a suitable biphasic system for laccase extraction.
Subject: Ciências Tecnológicas, Ciências da engenharia e tecnologias
Technological sciences, Engineering and technology
Scientific areas: Ciências da engenharia e tecnologias
Engineering and technology
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Programa de Financiamento Plurianual de Unidades de I&D/PEst-C/EQB/LA0020/2011/Projeto Estratégico-LA 20 - 2011-2012/LA 20
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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