Quantification of Caffeoylquinic Acids in Coffee Brews by HPLC-DAD

Abstract

The influence of different brewing conditions on the concentration of the main caffeoylquinic acids (3-caffeoylquinic acid (3-CQA),4-caffeoylquinic acid (4-CQA), and 5-caffeoylquinic acid (5-CQA)) was investigated. For this purpose, twenty-four coffee brewswere extracted and analyzed using HPLC-DAD at 325 nm. Our findings demonstrate the great impact of brewing techniques onthe caffeoylquinic acids (CQAs) content. The major isomer was 3-CQA, accounting for about 50% of the total CQAs, followed by 5-CQA and 4-CQA, accounting for about 24-36% for each one. The total content of CQAs was in the range of 45.79 to 1662.01 mg/L,found in iced cappuccino and pod espresso, respectively. In conclusion, this study demonstrates that coffee brews, in particularthose prepared using pressurized methods, can be considered as the potential sources of antioxidants such as CQAs.