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dc.creatorAlberto Domínguez
dc.creatorOscar Rodríguez
dc.creatorAna Paula M. Tavares
dc.creatorEugénia A. Macedo
dc.creatorMª Asunción Longo
dc.creatorMª Ángeles Sanromán
dc.description.abstractStability and kinetic behavior of laccase from Trametes versicolor in the presence of several ionic liquids from the methylimidazolium family have been investigated. In general laccase stability diminished as the size of the alkylic substitute in the methylimidazolium ring increased. Higher concentrations of ionic liquids caused more destabilization than lower ones. Thus, low concentrations of [C(2)mim(+)][EtSO(4)(-)] allowed maintaining enzymatic stability. [C(4)mim(+)][Cl(-)] appeared to have a stabilizing effect on laccase, as little activity decay was observed within three weeks. Kinetic studies indicated that both [C(2)mim(+)][EtSO(4)(-)] and [C(4)mim(+)][Cl(-)] inhibited laccase activity, although 10-fold more [C(2)mim(+)][EtSO(4)(-)] than [C(4)mim(+)][Cl(-)] was required to cause the same degree of inhibition. A kinetic model was developed to represent the experimental data.
dc.relationinfo:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Programa de Financiamento Plurianual de Unidades de I&D/PEst-C/EQB/LA0020/2011/Projeto Estratégico-LA 20 - 2011-2012/LA 20
dc.relationinfo:eu-repo/grantAgreement/CRUP - Conselho de Reitores das Universidades Portuguesas/Acções Integradas Luso-Espanholas/E-27/09/Ionic Liquids as Solvents for Enzymatic Catalysis, Acção Integrada Luso-Espanhola/E-27/09
dc.subjectCiências Tecnológicas, Ciências da engenharia e tecnologias
dc.subjectTechnological sciences, Engineering and technology
dc.titleStudies of laccase from Trametes versicolor in aqueous solutions of several methylimidazolium ionic liquids
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoFaculdade de Engenharia
dc.subject.fosCiências da engenharia e tecnologias
dc.subject.fosEngineering and technology
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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