Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/104694
Author(s): Ana Cristina Abreu
Maria José Saavedra
Lúcia C. Simões
Manuel Simões
Title: Combinatorial approaches with selected phytochemicals to increase antibiotic efficacy against Staphylococcus aureus biofilms
Issue Date: 2016
Abstract: Combinations of selected phytochemicals (reserpine, pyrrolidine, quinine, morin and quercetin)with antibiotics (ciprofloxacin, tetracycline and erythromycin) were tested on the prevention andcontrol of Staphylococcus aureus biofilms. The phytochemicals were also studied for their ability toavoid antibiotic adaptation and to inhibit antibiotic efflux pumps. Morin, pyrrolidine and quercetinat subinhibitory concentrations had significant effects in biofilm prevention and/or control whenapplied alone and combined with antibiotics. Synergism between antibiotics and phytochemicalswas found especially against biofilms of NorA overexpressing strain S. aureus SA1199B. This strainwhen growing with subinhibitory concentrations of ciprofloxacin developed increased tolerance tothis antibiotic. However, this was successfully reversed by quinine and morin. In addition, reserpineand quercetin showed significant efflux pump inhibition. The overall results demonstrate the role ofphytochemicals in co-therapies to promote more efficient treatments and decrease antimicrobialresistance to antibiotics, with substantial effects against S. aureus in both planktonic and biofilmstates.
URI: https://hdl.handle.net/10216/104694
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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