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Author(s): Pedro Carneiro
Joana Loureiro
Cristina Delerue Matos
Simone Morais
Maria do Carmo Pereira
Title: Alzheimer's disease: Development of a sensitive label-free electrochemical immunosensor for detection of amyloid beta peptide
Issue Date: 2017
Abstract: In this work, a highly sensitive label-free immunosensor for detection of the main biomarker ofAlzheimer's disease (AD), amyloid beta 1-42 (A (1-42)), is presented. A gold electrode was modifiedwith a mercaptopropionic acid (MPA) self-assembled monolayer, electrodeposited gold nanoparticles(AuNPs) and a monoclonal antibody mAb DE2B4 to recognize A; all the relevant experimental variableswere optimized. Antibodies were functionalized through chemical modification (thiolation) to promotethe antibody immobilization on the AuNPs surface with proper orientation which enabled the directdetection of A(1-42). Scanning electron microscopy, square-wave voltammetry and electrochemicalimpedance spectroscopy were used to characterize the construction of the biosensor. Using the proposed immunosensor, A(1-42) was specifically detected within the linear range of 10-1000 pg mL−1with a 5.2 pg mL−1 and 17.4 pg mL−1 detection and quantification limit, respectively; recovery values forthe tested spiking levels ranged from 90.3 to 93.6%. The immunosensor enables rapid, accurate, precise,reproducible and highly sensitive detection (14.6%reduction mL pg−1) of A with low-cost and opens thepossibilities for diagnostic ex vivo applications and research-based in vivo studies.
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/Referência: PTDC/QUI-BIQ/102827/2008/Concepção de péptidos fluorados para inibição da agregação do péptido beta-amilóide e avaliação da sua actividade biológica/Referência: PTDC/QUI-BIQ/102827/2008
info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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