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Author(s): Manuel Simões
Lúcia Chaves Simões
Nelson Lima
Title: Kinetics of biofilm formation by drinking water isolated Penicillium expansum
Issue Date: 2015
Abstract: Current knowledge on drinking water (DW) biofilms has been obtained mainly from studies on bacterial biofilms. Veryfew reports on filamentous fungi (ff ) biofilms are available, although they can contribute to the reduction in DW quality.This study aimed to assess the dynamics of biofilm formation by Penicillium expansum using microtiter plates under static conditions, mimicking water flow behaviour in stagnant regions of drinking water distribution systems. Biofilms wereanalysed in terms of biomass (crystal violet staining), metabolic activity (resazurin, fluorescein diacetate and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide [MTT]) and morphology (epifluorescence [calcofluor whiteM2R, FUN-1, FDA and acridine orange] and bright-field microscopies). Biofilm development over time showed the typical sigmoidal curve with noticeable different phases in biofilm formation (induction, exponential, stationary, and sloughing off ). The methods used to assess metabolic activity provided similar results. The microscope analysis allowedidentification of the involvement of conidia in initial adhesion (4 h), germlings (8 h), initial monolayers (12 h), a monolayer of intertwined hyphae (24 h), mycelial development, hyphal layering and bundling, and development of the maturebiofilms (≥48 h). P. expansum grows as a complex, multicellular biofilm in 48 h. The metabolic activity and biomass ofthe fungal biofilms were shown to increase over time and a correlation between metabolism, biofilm mass and hyphaldevelopment was found.
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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