Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/104482
Author(s): Ana Margarida Pereira
Ana Cristina Abreu
Manuel Simões
Title: Action of kanamycin against single and dual species biofilms of Escherichia coli and Staphylococcus aureus
Issue Date: 2012
Abstract: Antibiotic resistance in one of the greatest threats to mankind, and prospects for the discovery of new antibioticcompounds are reduced. Due to their low-level of usage, aminoglycosides seem to have remained active against manypathogens and thus gained a renewed interest. In this work, we tested the effects of kanamycin against single and dual speciesbiofilms of Escherichia coli and Staphylococcus aureus. The minimum inhibitory concentration (MIC), determined by themicrodilution method, of E. coli and S. aureus were 4.5 and 3.5 mg/L, respectively. The MIC was 5.0 mg/L for the dualspecies scenario. Biofilms were formed in 96-wells microtiter plates and their viability and biomass was assessed by alamarblue and crystal violet staining, respectively. Although the viability of sessile bacteria was greatly reduced with kanamycin(65% and 67% for E. coli and S. aureus, respectively), their removal from surfaces was more difficult (< 35%). For dualspecies biofilms, the highest viability reduction obtained was 70% and the removal percentage was 15%. Kanamycin wasmore efficient on biofilm killing than on their removal. Total biofilm killing and removal was not achieved even for kanamycinat 10 × MIC and resistance was more pronounced for the dual species biofilms. This highlights the greatest resistancecapacity of biofilms when compared with planktonic cells. The overall results suggest that improved therapeutic strategies,including the application of higher doses or the combination with synergistic products, should be applied in biofilm control,particularly in polymicrobial biofilms.
Subject: Ciências médicas e da saúde
Medical and Health sciences
Scientific areas: Ciências médicas e da saúde
Medical and Health sciences
URI: https://repositorio-aberto.up.pt/handle/10216/104482
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/PTDC/EBB-EBI/105085/2008/Estudo da influência do fenótipo de biofilmes na sua resiliência e resistência/Bioresist
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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