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Author(s): Joana Teodósio
M. Simões
F. J. Mergulhão
Title: The influence of nonconjugative Escherichia coli plasmids on biofilm formation and resistance
Issue Date: 2012
Abstract: Aims: This work describes the effects of the presence of nonconjugative plasmids in Escherichia coli cells forming biofilms on a flow cell system under turbulent conditions. Methods and Results: The pET28 and pUC8 plasmids were separately used to transform E.similar to coli JM109(DE3). Biofilm formation, removal and antimicrobial susceptibility to the cationic biocide benzyldimethyldodecylammonium chloride (BDMDAC) were assessed. Transformed cells formed thicker biofilms with higher cell densities, and the metabolic activity was higher whereas nontransformed cells had higher viabilities. Biocide treatment was not efficient for biofilm removal but was effective for cell killing. Biofilms formed by nontransformed cells were less affected by the treatment. Conclusions: Cell transformation with the tested plasmids has significant impacts on biofilm formation, cell viability, metabolic activity and resistance to biocide treatment. Our results show that in biofilm studies involving deletion/complementation experiments, a control with the strain carrying a plasmid devoid of the gene under investigation must be included so that the real effects of the genetic manipulation are not biased by the presence of the plasmid backbone. Significance and Impact of the Study: This is the first report where the presence of nonconjugative plasmids is assessed in flow conditions analysing biofilm formation, removal and antimicrobial susceptibility of high cell-density biofilms.
Subject: Ciências biológicas
Biological sciences
Scientific areas: Ciências exactas e naturais::Ciências biológicas
Natural sciences::Biological sciences
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/PTDC/BIO/69092/2006/Estudo das interacções entre a secreção de proteínas recombinantes e a formação e estabilidade de biofilmes/PTDC/BIO/69092/2006
info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Programa MIT-Portugal/MIT-Pt/BS-BB/0082/2008/Biologia de sistemas e Biologia sintética no desenvolvimento de fábricas celulares microbianas melhoradas/MIT-Pt/BS-BB/0082/2008
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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