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Author(s): Luciana C. Gomes
Joana M. R. Moreira
Manuel Simões
Luís F. Melo
Filipe J. Mergulhão
Title: Biofilm Localization in the Vertical Wall of Shaking 96-Well Plates
Issue Date: 2014
Abstract: Microtiter plates with 96 wells are being increasingly used for biofilm studies due to their high throughput, low cost, easy handling,and easy application of several analytical methods to evaluate different biofilm parameters.These methods provide bulk informationabout the biofilm formed in each well but lack in detail, namely, regarding the spatial location of the biofilms. This location canbe obtained by microscopy observation using optical and electron microscopes, but these techniques have lower throughput andhigher cost and are subjected to equipment availability. This work describes a differential crystal violet (CV) staining method thatenabled the determination of the spatial location of Escherichia coli biofilms formed in the vertical wall of shaking 96-well plates.It was shown that the biofilms were unevenly distributed on the wall with denser cell accumulation near the air-liquid interface.The results were corroborated by scanning electron microscopy and a correlation was found between biofilm accumulation and thewall shear strain rates determined by computational fluid dynamics. The developed method is quicker and less expensive and hasa higher throughput than the existing methods available for spatial location of biofilms in microtiter plates.
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/PTDC/EBB-BIO/102863/2008/Construção de bactérias "terapêuticas" usando uma abordagem de biologia sintética/SYNBIOBACTHER
info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/PTDC/EBB-BIO/104940/2008/Estudo das condições iniciais que controlam a formação de biofilmes/Filmstart
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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