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Author(s): Joana Azeredo
Nuno F. Azevedo
Romain Briandet
Nuno Cerca
Tom Coenye
Ana Rita Costa
Michaël Desvaux
Giovanni Di Bonaventura
Michel Hébraud
Zoran Jaglic
Miroslava Kacániová
Susanne Knochel
Anália Lourenço
Filipe Mergulhão
Rikke Louise Meyer
George Nychas
Manuel Simões
Odile Tresse
Claus Sternberg
Title: Critical review on biofilm methods
Issue Date: 2017
Abstract: Biofilms are widespread in nature and constitute an important strategy implemented by microorganisms to survive in sometimes harsh environmental conditions. They can be beneficial or havea negative impact particularly when formed in industrial settings or on medical devices. As such,research into the formation and elimination of biofilms is important for many disciplines. Severalnew methodologies have been recently developed for, or adapted to, biofilm studies that havecontributed to deeper knowledge on biofilm physiology, structure and composition. In thisreview, traditional and cutting-edge methods to study biofilm biomass, viability, structure, composition and physiology are addressed. Moreover, as there is a lack of consensus among thediversity of techniques used to grow and study biofilms. This review intends to remedy this, bygiving a critical perspective, highlighting the advantages and limitations of several methods.Accordingly, this review aims at helping scientists in finding the most appropriate and up-to-datemethods to study their biofilms.
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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