Please use this identifier to cite or link to this item:
Author(s): J. Malheiro
Paula Araújo
Idalina Machado
Madalena Lemos
Filipe Mergulhão
L. F. Melo
Simões M
Title: The Effects of Selected Brominated and Chlorinated Chemicals on Pseudomonas fluorescens Planktonic Cells and Flow-Generated Biofilms
Issue Date: 2016
Abstract: Sodium hypochlorite (SH) is a widely used disinfectant. However, due to public health and environmental concerns, alternative disinfectants are required. In this study, the control of Pseudomonas fluorescens planktonic and biofilm cells was assessed with cetyltrimethylammonium bromide (CTAB), SH, 3-bromopropionyl chloride (BPC) and 3-bromopropionic acid (BPA). Their effects were tested against planktonic cells in terms of antimicrobial efficiency and changes on cell surface. CTAB had the lowest minimum inhibitory concentration or minimum bactericidal concentration (20/50g/mL). All chemicals promoted cellular disruption and leakage of intracellular constituents. CTAB, BPC and BPA irreversibly changed surface hydrophobicity and charge. Their action was also assessed in the control of flow-generated biofilms. Modest killing and removal was achieved (maximum killing of 1log and 16% of total biofilm removal) due to the characteristics of the biofilm. In general, both BPC and CTAB had antimicrobial activities similar to SH and therefore demonstrated potential as alternatives to SH in P.fluorescens control. Practical ApplicationsThe potential environmental and health problems caused by the uncontrolled use of sodium hypochlorite (SH) have led to the search for alternatives, maintaining the antimicrobial efficacy. In fact, SH is included in the indicative list of the Directive on Industrial Emissions (2010/75/EU) as a major pollutant for water emissions and on the formation of carcinogenic and mutagenic products in the presence of organic matter. Therefore, in this study, the antimicrobial potential of selected halogen-based products was assessed in order to compare their efficacy with SH in the control of planktonic and biofilm cells. All products caused apparent membrane pore formation as well as hydrophobicity and surface charge modification against planktonic cells. Additionally, 3-bromopropionyl chloride and cetyltrimethylammonium bromide demonstrated similar action to SH in the control of biofilms mimicking an industrial scenario.
Related Information: info:eu-repo/grantAgreement/Autoriadade de Gestão do Programa Operacional Regional do Norte/Programas Integrados de IC&DT/NORTE-07-0124-FEDER-000025/(Bio) Chemical Engineering: Multi-Scale Approaches for Sustainable Environment and Health/LEPAE/CEFT - RL2
info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE
info:eu-repo/grantAgreement/Autoridade de Gestão do Programa Operacional de Cooperação Transfronteiriça Espanha-Portugal/Programa Operacional de Cooperação Transfronteiriça Espanha-/0687_NOVOMAR_1_P/Consolidação do Centro Multipolar de Valorização de Recursos Marinhos visando novos usos do mar/NOVOMAR
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

Files in This Item:
File Description SizeFormat 
  Restricted Access
Artigo original publicado410.11 kBAdobe PDF    Request a copy from the Author(s)
144264.1.pdfPost-Print version513.03 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.