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Author(s): Joana M. R.Moreira
Luciana C. Gomes
José D. P. Araújo
João M. Miranda
Manuel Simões
Luís F. Melo
Filipe J. Mergulhão
Title: The effect of glucose concentration and shaking conditions on Escherichia coli biofilm formation in microtiter plates
Issue Date: 2013
Abstract: Microtiter plates are one of the most widely used platforms for biofilm studies. However, these systems have been used rather blindly without knowledge of the flow characteristics inside them. Plates with 96-wells were used to study flow dynamics and its influence on Escherichia coli JM109(DE3) biofilm formation. Two different glucose concentrations (1 g L-1 and 0.25 g L-1) and two different shaking conditions were tested using incubators with orbital diameters of 50 and 25 mm. Biofilm growth was monitored for 60 h and the hydrodynamics were simulated by computational fluid dynamics. These simulations have shown that wall shear stress was higher near the air/liquid interface and average values of 0.070 and 0.034 Pa were obtained for the largest and smallest orbital diameter. For the high glucose concentration, the maximum biofilm amount was attained at 24 h and similar values were obtained in both incubators. For the low glucose concentration, lower values were attained. Numerical simulations indicated that microtiter plates can adequately model biofilm formation in relevant biomedical systems.
Subject: Engenharia química
Chemical engineering
Scientific areas: Ciências da engenharia e tecnologias::Engenharia química
Engineering and technology::Chemical engineering
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/PTDC/EBB-BIO/102863/2008/Construção de bactérias "terapêuticas" usando uma abordagem de biologia sintética/SYNBIOBACTHER
info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/PTDC/EBB-BIO/104940/2008/Estudo das condições iniciais que controlam a formação de biofilmes/Filmstart
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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