Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/103582
Author(s): | José M. Sousa Gonçalo Macedo Marta Pedrosa Cristina Becerra Castro Sérgio Castro Silva M. Fernando R. Pereira Adrián M. T. Silva Olga C. Nunes Célia M. Manaia |
Title: | Ozonation and UV254 nm radiation for the removal of microorganisms and antibiotic resistance genes from urban wastewater |
Issue Date: | 2017 |
Abstract: | Conventional wastewater treatment has a limited capacity to reduce antibiotic resistant bacteria and genes (ARB&ARG). Tertiary treatment processes are promising solutions, although the transitory inactivation of bacteria may select ARB&ARG. This study aimed at assessing the potential of ozonation and UV254 nm radiation to inactivate cultivable fungal and bacterial populations, and the selected genes 16S rRNA (common to all bacteria), intl1 (common in Gram-negative bacteria) and the ARG vanA, bIa(TEM), sul1 and qnrS. The abundance of the different microbiological parameters per volume of wastewater was reduced by similar to 2 log units for cultivable fungi and 16S rRNA and intll genes, by similar to 3-4 log units, for total heterotrophs, enterobacteria and enterococci, and to values close or below the limits of quantification for ARG, for both processes, after a contact time of 30 min. Yet, most of the cultivable populations, the 16S rRNA and intll genes as well as the ARG, except qnrS after ozonation, reached pre-treatment levels after 3 days storage, suggesting a transitory rather than permanent microbial inactivation. Noticeably, normalization per 16S rRNA gene evidenced an increase of the ARG and intl1 prevalence, mainly after UV254 (nm) treatment. The results suggest that these tertiary treatments may be selecting for ARB&ARG populations. |
URI: | https://hdl.handle.net/10216/103582 |
Related Information: | info:eu-repo/grantAgreement/Autoridade de Gestão do Programa Operacional Regional do Norte/Programas Integrados de IC&DT/NORTE-07-0124-FEDER-000025/(Bio) Chemical Engineering: Multi-Scale Approaches for Sustainable Environment and Health/LEPAE/CEFT - RL2 info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e a Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE info:eu-repo/grantAgreement/Agência de Inovação, S.A./Projectos de I&DT em Co-Promoção/ SI IDT - 38900/2012 F3 /Novo equipamento envolvendo processos catalíticos integrados para tratamento de poluentes orgânicos e desinfeção de águas/NEPCAT info:eu-repo/grantAgreement/COMISSÃO EUROPEIA/COST - European Cooperation in Science and Technology/ES1403PT//NEREUS |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | restrictedAccess |
Appears in Collections: | FEUP - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
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142432.pdf Restricted Access | Artigo original publicado | 723.81 kB | Adobe PDF | Request a copy from the Author(s) |
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