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Author(s): Ana Abreu
Rita Serra
Anabela Borges
Maria José Saavedra
António Salgado
Manuel Simões
Title: Evaluation of the best method to assess antibiotic potentiation by phytochemicals against Staphylococcus aureus
Issue Date: 2014
Abstract: The increasing occurrence of bacterial resistance to antibiotics has now reached a critical level. Finding antibiotic coadjuvants capable to inhibit the bacterial resistance mechanisms would be a valuable mid-term solution, until new classes of antibiotics are discovered. Selected plant alkaloids were combined with 5 antibiotics against 10 Staphylococcus aureus strains, including strains expressing distinct efflux pumps and methicillin-resistant S. aureus strains. The efficacy of each combination was assessed using the microdilution checkerboard, time-kill, Etest, and disc diffusion methods. The cytotoxicity of the alkaloids was evaluated in a mouse fibroblast cell line. Potentiation was obtained in 6% of all 190 combinations, especially with the combination of: ciprofloxacin with reserpine (RES), pyrrolidine (PYR), and quinine (QUIN); tetracycline with RES; and erythromycin with PYR. The highest cytotoxicity values were found for QUIN (half maximal inhibitory concentration [IC50] = 25 +/- 2.2 mg/L) and theophylline (IC50 = 100 +/- 4.7 mg/L).
Subject: Ciências da Saúde, Ciências da engenharia e tecnologias
Health sciences, Engineering and technology
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/PTDC/DTP-SAP/1078/2012|FCOMP-01-0124-FEDER-028765/Desinfeção de bactérias planctónicas e biofilmes em ambientes hospitalares com produtos fitoquímicos/Fitodesinfetantes
info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/PTDC/EBB-EBI/105085/2008/Estudo da influência do fenótipo de biofilmes na sua resiliência e resistência/Bioresist
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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