Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/103289
Author(s): | Ana Cristina Abreu Sofia C. Serra Anabela Borges Maria José Saavedra António J. Salgado Manuel Simões |
Title: | Evaluation of the best method to assess antibiotic potentiation by phytochemicals against Staphylococcus aureus |
Issue Date: | 2014 |
Abstract: | The increasing occurrence of bacterial resistance to antibiotics has now reached a critical level. Finding antibiotic coadjuvants capable to inhibit the bacterial resistance mechanisms would be a valuable mid-term solution, until new classes of antibiotics are discovered. Selected plant alkaloids were combined with 5 antibiotics against 10 Staphylococcus aureus strains, including strains expressing distinct efflux pumps and methicillin-resistant S. aureus strains. The efficacy of each combination was assessed using the microdilution checkerboard, time-kill, Etest, and disc diffusion methods. The cytotoxicity of the alkaloids was evaluated in a mouse fibroblast cell line. Potentiation was obtained in 6% of all 190 combinations, especially with the combination of: ciprofloxacin with reserpine (RES), pyrrolidine (PYR), and quinine (QUIN); tetracycline with RES; and erythromycin with PYR. The highest cytotoxicity values were found for QUIN (half maximal inhibitory concentration [IC50] = 25 +/- 2.2 mg/L) and theophylline (IC50 = 100 +/- 4.7 mg/L). |
Subject: | Ciências da Saúde, Ciências da engenharia e tecnologias Health sciences, Engineering and technology |
Scientific areas: | Ciências da engenharia e tecnologias Engineering and technology |
URI: | https://hdl.handle.net/10216/103289 |
Related Information: | info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e a Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/PTDC/EBB-EBI/105085/2008/Estudo da influência do fenótipo de biofilmes na sua resiliência e resistência/Bioresist info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e a Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/PTDC/DTP-SAP/1078/2012|FCOMP-01-0124-FEDER-028765/Desinfeção de bactérias planctónicas e biofilmes em ambientes hospitalares com produtos fitoquímicos/Fitodesinfetantes |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | restrictedAccess |
Appears in Collections: | FEUP - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
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105695.pdf Restricted Access | Artigo original publicado | 584.88 kB | Adobe PDF | Request a copy from the Author(s) |
105695.1.pdf | Post-Print version | 1.05 MB | Adobe PDF | View/Open |
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