Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/103253
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dc.creatorSilvia Castro Coelho
dc.creatorMaria do Carmo Pereira
dc.creatorcoelho, man
dc.creatorGabriela M. Almeida
dc.creatorFilipe Santos-Silva
dc.date.accessioned2019-02-01T01:49:59Z-
dc.date.available2019-02-01T01:49:59Z-
dc.date.issued2016-08
dc.identifier.issn1742-5247
dc.identifier.othersigarra:144821
dc.identifier.urihttps://repositorio-aberto.up.pt/handle/10216/103253-
dc.description.abstractObjectives: Gold nanoparticles have become promising vectors for cancer diagnosis and treatment. The present study investigates the effect of bortezomib (BTZ), a proteasome inhibitor, conjugated with pegylated gold nanoparticles (PEGAuNPs) in pancreatic and lung cancer cells. Methods: Synthesized gold nanoparticles (PEGAuNPs) were conjugated with bortezomib antitumor drug. We investigated the cytotoxicity induced by BTZ conjugated with functionalized gold nanoparticles in vitro, in the human pancreatic (S2-013) and lung (A549) cancer cell lines. Results: We found an efficient of conjugation of BTZ with PEGAuNPs. In vitro assays showed that after 72 h' incubation with PEGAuNPs-BTZ cancer cells revealed alterations in morphology; also for S2-013 and A549 cancer cells, the IC50 value of free BTZ is respectively 1.5 and 4.3 times higher than the IC50 value of PEGAuNPs-BTZ. Furthermore, for TERT-HPNE, the IC50 value is around 63 times lower for free BTZ than the conjugated nanovehicle. Cell growth inhibition results showed a remarkable enhancement in the effect of BTZ when conjugated with AuNPs. Conclusions: Our findings showed that conjugation with PEGAuNPs enhance the BTZ growth-inhibition effect on human cancer cells (S2-013 and A549) and decreases its toxicity against normal cells (TERT-HPNE).
dc.language.isoeng
dc.relationinfo:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE
dc.relationinfo:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de Cooperação Internacional/TRANSCAN/0001/2012/Nanoparticle enhanced molecular Fluorescence-Endoscopy for detection of early stage colorectal adenocarcinoma/NanoEFEct
dc.rightsrestrictedAccess
dc.subjectNanotecnologia
dc.subjectNano-technology
dc.titleEnhancing the efficiency of bortezomib conjugated to pegylated gold nanoparticles: an in vitro study on human pancreatic cancer cells and adenocarcinoma human lung alveolar basal epithelial cells
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoFaculdade de Engenharia
dc.identifier.doi10.1080/17425247.2016.1178234
dc.identifier.authenticusP-00K-RE1
dc.subject.fosCiências da engenharia e tecnologias::Nanotecnologia
dc.subject.fosEngineering and technology::Nano-technology
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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