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Author(s): J. M. R. Moreira
L. C. Gomes
K. A. Whitehead
S. Lynch
L. A. Tetlow
F. J. Mergulhão
Title: Effect of surface conditioning with cellular extracts on Escherichia coli adhesion and initial biofilm formation
Issue Date: 2017
Abstract: Bacterial adhesion and subsequent biofilm formation start with surface conditioning bymolecules originating from the surrounding medium and from cell lysis. Different cellextracts e.g. total cell extract (TCE), cytoplasm with cellular debris (CCDE) and periplasmic extract (PE) were tested in agitated 96-well microtiter plates and in a flow cell. Crystalviolet assay demonstrated that a polystyrene substratum conditioned with TCE or CCDEdecreased initial biofilm formation, however cell adhesion generally increased when PE wasused. These results were dependent on conditioning film concentration. Using a parallelplate flow chamber, the use of optimal conditioning film concentrations resulted in all thedifferent cellular extracts reducing biofilm formation. Multifractal analysis was used to generate quantitative data on the number of cell clusters. Surface conditioning with cellularcomponents affected the amount and clustering of bacteria on polystyrene surfaces andtheir propensity to induce biofilm formation. To the best of our knowledge, this is the firststudy addressing the effect of cellular surface conditioning of cellular compartments onEscherichia coli adhesion and initial biofilm formation. This work leads to a greater understanding of the factors that influence biofilm formation under flow conditions which areprevalent in food industry.(c) 2017 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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