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Author(s): Sílvia Fontenete
Nuno Guimarães
Jesper Wengel
Nuno Filipe azevedo
Title: Prediction of melting temperatures in fluorescencein situhybridization (FISH) procedures using thermodynamic models
Issue Date: 2016
Abstract: The thermodynamics and kinetics of DNA hybridization, i.e. the process of self-assembly ofone, two or more complementary nucleic acid strands, has been studied for many years. Theappearance of the nearest-neighbor model led to several theoretical and experimental paperson DNA thermodynamics that provide reasonably accurate thermodynamic informationon nucleic acid duplexes and allow estimation of the melting temperature. Because thereare no thermodynamic models specifically developed to predict the hybridization temperatureof a probe used in a fluorescence in situ hybridization (FISH) procedure, the meltingtemperature is used as a reference, together with corrections for certain compounds that areused during FISH. However, the quantitative relation between melting and experimental FISHtemperatures is poorly described. In this review, various models used to predict the meltingtemperature for rRNA targets, for DNA oligonucleotides and for nucleic acid mimics (chemicallymodified oligonucleotides), will be addressed in detail, together with a critical assessment ofhow this information should be used in FISH.
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de Investigação Clínica/PIC/IC/82815/2007/Desenvolvimento e aplicação de mímicos de DNA para a rápida identificação de microrganismos patogénicos/DNA mimics
info:eu-repo/grantAgreement/Autoriadade de Gestão do Programa Operacional Regional do Norte/Programas Integrados de IC&DT/NORTE-07-0124-FEDER-000025/(Bio) Chemical Engineering: Multi-Scale Approaches for Sustainable Environment and Health/LEPAE/CEFT - RL2
info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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