Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/102962
Author(s): | Berta N. Estevinho Irena Ramos Fernando Rocha |
Title: | Effect of the pH in the formation of beta-galactosidase microparticles produced by a spray-drying process |
Issue Date: | 2015 |
Abstract: | The objective of this work was to investigate the influence of pH in the microencapsulation process, using a modified chitos an to microencapsulate the enzyme beta-galactosidase, by a spray-drying technique. Structural analysis of the surface of the particles was performed by scanning electron microscopy (SEM), showing that the obtained microparticles have an average diameter smaller than 3.5 mu m and in general a regular shape. The activity of the enzyme was studied by spectrophotometric methods using the substrate P-nitrophenyl-beta,D-galactopyranoside (ONPG). The parameters of Michaelis-Menten were calculated. The value of K-m decreases with the decrease of the pH, which can be associated to an increase of the affinity between the enzyme and substrate to smaller pH's. The highest value of the parameter V-max, representing the maximum reaction rate at a given enzyme concentration, was obtained at pH 6. |
Subject: | Ciências Tecnológicas, Ciências biológicas Technological sciences, Biological sciences |
Scientific areas: | Ciências exactas e naturais::Ciências biológicas Natural sciences::Biological sciences |
URI: | https://hdl.handle.net/10216/102962 |
Related Information: | info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e a Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | restrictedAccess |
Appears in Collections: | FEUP - Artigo em Revista Científica Internacional |
Files in This Item:
File | Description | Size | Format | |
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102173.1.pdf | Post-Print version | 1.15 MB | Adobe PDF | View/Open |
102173.pdf Restricted Access | Artigo original publicado | 1.28 MB | Adobe PDF | Request a copy from the Author(s) |
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