Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/102899
Author(s): Andreia S. Azevedo
Carina Almeida
Luciana C. Gomes
Filipe J. Mergulhão
Luís F. Melo
Nuno F. Azevedo
Carla Ferreira
Title: An in vitro model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium
Issue Date: 2017-02-15
Abstract: Uncommon bacteria, such as Delftia tusurhatensis have been isolated from CAUTIs in combination with well-established pathogenic bacteria such as Escherichia colt. Nonetheless, the reason why E. colt coexists with other bacteria instead of outcompeting and completely eliminating them is unknown. As such, a flow cell reactor simulating the hydrodynamic conditions found in CAUTIs (shear rate of 15 s(-1)) was used to characterize the microbial physiology of E. coli and D. tsuruhatensis individually and in consortium, in terms of the growth kinetics and substrate uptake. Single-species biofilms showed that up to 48 h the cultivable cell counts significantly increased for both species (p < 0.05). When in dual-species biofilm, E. coli outnumbered D. tsuruhatensis up to 16 h and then D. tsuruhatensis gained a fitness advantage. However, the assessment of the spatial distribution of the dual-species biofilm by LNA/2'OMe-FISH revealed that E. coli and D. tsuruhatensis coexist and tend to co-aggregate over time, which suggests that both bacteria are able to cooperate synergistically. Substrate uptake measurements revealed that D. tsuruhatensis metabolized citric acid more rapidly, presumably leaving more uric acid available in the medium to be used by E. colt. In conclusion, E. colt and uncommon bacteria seem to cooperate, when sharing the same environment under dynamic conditions, leading to the persistence of both bacteria in a stable microbial community.
URI: https://hdl.handle.net/10216/102899
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de Investigação Clínica/PIC/IC/82815/2007/Desenvolvimento e aplicação de mímicos de DNA para a rápida identificação de microrganismos patogénicos/DNA mimics
info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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