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Author(s): Berta N. Estevinho
Ana M. Damas
Pedro Martins
Fernando Rocha
Title: Study of the inhibition effect on the microencapsulated enzyme β-Galactosidase
Issue Date: 2012
Abstract: The aim of this work is to investigate the influence of an inhibitor over free and microencapsulated enzymes. Microencapsulation is one of the techniques for enzyme immobilization, which is a very common process in the industry to stabilize enzymes and to increase the time and the range of applicability. There are several microencapsulation techniques, with different adaptations and specificities. In this work we used a spray drying method which is a technology used in industry due to its low cost, availability of equipment and efficiency. Spray drying is also a clean technology because it avoids the use of organic solvents during the encapsulation process. This study focused on the enzyme beta-galactosidase, due to its importance in health and industry. Chitosan was selected as encapsulating agent considering all the advantages of this natural polymer. The microcapsules were prepared by spray drying and characterized by their particle size and surface morphology. Structural analysis of the surface of the particles was performed by Scanning Electron Microscopy (SEM). The SEM results show that the obtained microcapsules have a diameter smaller than 5 mu m and a regular shape. The activity of the enzyme was studied by a spectrophotometric method using the substrate ONPG (O-Nitrophenyl-beta, D-galactopyranoside) and MGP (methyl-beta, D-galactopyranosidase) as an inhibitor. It was concluded that there are differences on beta-galactosidase activity in the presence of the inhibitor. The results also showed that the use of an encapsulating agent increases the diffusional effect of the released enzyme, and also reduces the initial activity of the enzyme.
Subject: Ciências da terra e ciências do ambiente
Earth and related Environmental sciences
Scientific areas: Ciências exactas e naturais::Ciências da terra e ciências do ambiente
Natural sciences::Earth and related Environmental sciences
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Programa de Financiamento Plurianual de Unidades de I&D/FCOMP-01-0124-FEDER-022677/PROJECTO ESTRATÉGICO - UI 511 - 2011-2012/PEst-C/EQB/UI0511/2011
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional
ICBAS - Artigo em Revista Científica Internacional

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