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Author(s): Maria do Carmo Pereira
Diana Paiva
Thomas Markowski
Bodo Dobner
Gerald Brezesinski
Helmuth Möhwald
Sandra Rocha
Title: Synthesis and study of the complex formation of a cationic alkyl-chain bola amino alcohol with DNA: in vitro transfection efficiency
Issue Date: 2015
Abstract: Recent studies point out that bolaamphiphiles can be used in nonviral gene therapy. Due to their bipolar character, they may span a membrane and thus stabilize or destabilize it which could be relevant for DNA transfer across a biological membrane. Since there are only very few studies on bolaamphiphile application in DNA transfection, it is difficult to assess whether they will bring additional advantages to the class of nonviral vectors. A bolaamphiphile with a hydrophobic chain of 22 carbon atoms with trimethylammonium and hydroxyl groups at each end was synthesized (22-hydroxydocos-1-yl-N,N,N-trimethylammonium bromide). The bolaamphiphile conferred stability and lowered the zeta potential of vesicles of a cationic lipid. Confocal laser scanning microscopy and flow cytometry experiments have shown that this bolaamphiphile can transfect the green fluorescent protein plasmid in cells if mixed with a cationic lipid. It can compete with standard cationic lipids in terms of transfection but is at the same time less toxic. The potential of this class of molecules in gene delivery results from the fact that they will confer high stability to the DNA vector.
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/PEst-C/EQB/UI0511/2013/PROJECTO ESTRATÉGICO - UI 511 - 2013-2014/UI0511
info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/Referência: PTDC/QUI-BIQ/102827/2008/Concepção de péptidos fluorados para inibição da agregação do péptido beta-amilóide e avaliação da sua actividade biológica/Referência: PTDC/QUI-BIQ/102827/2008
info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/PTDC/QUI-BIQ/118076/2010/Liposomas funcionalizados com anticorpos para a doença de Alzheimer/PTDC/QUI-BIQ/118076/2010
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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