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Author(s): Diana Paiva
Albertoo Martín-Molina
Isabel Cardoso
Manuel Quesada-Pérez
Maria do Carmo Pereira
Sandra Rocha
Title: The effect of a fluorinated cholesterol derivative on the stability and physical properties of cationic DNA vectors
Issue Date: 2013
Abstract: Liposomes of the cationic lipid DOTAP (1,2-dioleoyl-3-trimethylammonium-propane) and the fluorinated cholesterol derivative heptafluorocholesterol (F7-CHOL) were tested at the molar ratio of 1 : 1 for DNA compaction and transfection. Their properties were correlated with the characteristics of the well-known system, DOTAP and cholesterol (CHOL). The mass lipid-DNA (L/D) ratios at the isoelectric point were within the ranges 3-4 for DOTAP: CHOL and 4-5 for DOTAP: F7-CHOL, as determined by electrophoretic mobility measurements. These results and the ethidium bromide fluorescence intercalation assays confirmed that more DOTAP: F7-CHOL liposomes are needed to compact the same amount of DNA as DOTAP: CHOL. The phase diagrams of aggregation and re-entrant condensation phenomena obtained by phenomenological theory support this conclusion and also establish that the liposome-DNA binding is stronger in the case of the DOTAP:F7-CHOL-DNA system. The stability rates of both liposomes in the presence of DNA and their transfection efficiencies were similar, which support the application of heptafluorocholesterol as a helper lipid in cationic liposomes for DNA delivery.
Subject: Ciências da Saúde, Ciências Tecnológicas, Engenharia dos materiais
Health sciences, Technological sciences, Materials engineering
Scientific areas: Ciências da engenharia e tecnologias::Engenharia dos materiais
Engineering and technology::Materials engineering
Related Information: info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/Referência: PTDC/QUI-BIQ/102827/2008/Concepção de péptidos fluorados para inibição da agregação do péptido beta-amilóide e avaliação da sua actividade biológica/Referência: PTDC/QUI-BIQ/102827/2008
info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/PTDC/QUI-BIQ/118076/2010/Liposomas funcionalizados com anticorpos para a doença de Alzheimer/PTDC/QUI-BIQ/118076/2010
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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