Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10216/91493
Autor(es): | Ascensao, A Magalhaes, J Soares, JMC Ferreira, R Neuparth, MJ Marques, F Oliveira, PJ Duarte, JA |
Título: | Moderate endurance training prevents doxorubicin-induced in vivo mitochondriopathy and reduces the development of cardiac apoptosis |
Data de publicação: | 2005 |
Resumo: | The objective of this work was to test the hypothesis that endurance training may be protective against in vivo doxorubicin (DOX)-induced cardiomyopathy through mitochondria-mediated mechanisms. Forty adult (6-8 wk old) male Wistar rats were randomly divided into four groups (n=10/group): nontrained, nontrained + DOX treatment (20 mg/kg), trained (14 wk of endurance treadmill running, 60-90 min/day), and trained + DOX treatment. Mitochondrial respiration, calcium tolerance, oxidative damage, heat shock proteins (HSPs), antioxidant enzyme activity, and apoptosis markers were evaluated. DOX induces mitochondrial respiratory dysfunction, oxidative damage, and histopathological lesions and triggers apoptosis (P<0.05, n=10). However, training limited the decrease in state 3 respiration, respiratory control ratio (RCR), uncoupled respiration, aconitase activity, and protein sulfhydryl content caused by DOX treatment and prevented the increased sensitivity to calcium in nontrained + DOX- treated rats (P<0.05, n=10). Moreover, training inhibited the DOX- induced increase in mitochondrial protein carbonyl groups, malondialdehyde, Bax, Bax-to-Bcl-2 ratio, and tissue caspase-3 activity (P<0.05, n=10). Training also increased by similar to 2-fold the expression of mitochondrial HSP-60 and tissue HSP-70 (P<0.05, n=10) and by similar to 1.5-fold the activity of mitochondrial and cytosolic forms of SOD (P<0.05, n=10). We conclude that endurance training protects heart mitochondrial respiratory function from the toxic effects of DOX, probably by improving mitochondrial and cell defense systems and reducing cell oxidative stress. In addition, endurance training limited the DOX- triggered apoptosis. |
Assunto: | Medicina clínica Clinical medicine |
Áreas do conhecimento: | Ciências médicas e da saúde::Medicina clínica Medical and Health sciences::Clinical medicine |
URI: | https://hdl.handle.net/10216/91493 |
Tipo de Documento: | Artigo em Revista Científica Internacional |
Condições de Acesso: | restrictedAccess |
Aparece nas coleções: | FADEUP - Artigo em Revista Científica Internacional FFUP - Artigo em Revista Científica Internacional |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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45287.pdf Restricted Access | 595.19 kB | Adobe PDF | Request a copy from the Author(s) |
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