Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10216/53558
Autor(es): | Maffini, S Maia, ARR Manning, AL Maliga, Z Pereira, AL Junqueira, M Shevchenko, A Hyman, A Yates, JR Galjart, N Compton, DA Maiato, H |
Título: | Motor-Independent Targeting of CLASPs to Kinetochores by CENP-E Promotes Microtubule Turnover and Poleward Flux |
Outro Título: | CENP-E targeting of CLASPs to kinetochores |
Data de publicação: | 2009 |
Resumo: | Efficient chromosome segregation during mitosis relies on the coordinated activity of molecular motors with proteins that regulate kinetochore attachments to dynamic spindle microtubules [1]. CLASPs are conserved kinetochore- and microtubule-associated proteins encoded by two paralogue genes, clasp1 and clasp2, and have been previously implicated in the regulation of kinetochore-microtubule dynamics [2-4]. However, it remains unknown how CLASPs work in concert with other proteins to form a functional kinetochore-microtubule interface. Here we have identified mitotic interactors of human CLASP1 using a proteomic approach. Among these, the microtubule plus-end directed motor CENP-E [5] was found to form a complex with CLASP1 that co-localizes to multiple structures of the mitotic apparatus in human cells. We found that CENP-E recruits both CLASP1 and CLASP2 to kinetochores independent of its motor activity or the presence of microtubules. Depletion of CLASPs or CENP-E by RNAi in human cells causes a significant and comparable reduction of kinetochore-microtubule poleward flux and turnover rates, as well as rescues spindle bipolarity in Kif2a-depleted cells. We conclude that CENP-E integrates two critical functions that are important for accurate chromosome movement and spindle architecture: one relying directly on its motor activity and the other involving the targeting of key microtubule regulators to kinetochores. |
Assunto: | Flux Kinetochores Microtubule Turnover Mitosis Mitotic Spindle |
URI: | http://hdl.handle.net/10216/53558 |
Ligação ao Catálogo: | http://dx.doi.org/10.1016/j.cub.2009.07.059 |
Fonte: | Current Biology, vol.19(18), 1566-1572, |
Tipo de Documento: | Artigo em Revista Científica Internacional |
Condições de Acesso: | openAccess |
Aparece nas coleções: | I3S - Artigo em Revista Científica Internacional |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Motor-Independent Targeting of CLASPs to Kinetochores by CENP-E Promotes Microtubule Turnover and Poleward Flux.pdf | 18.43 MB | Adobe PDF | Ver/Abrir |
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