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https://hdl.handle.net/10216/1911
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DC Field | Value | Language |
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dc.creator | Maria Maia | |
dc.creator | Paula Serrão | |
dc.creator | Maria Manuela Moura | |
dc.creator | Manuel Pestana | |
dc.date.accessioned | 2022-09-12T04:56:47Z | - |
dc.date.available | 2022-09-12T04:56:47Z | - |
dc.date.issued | 2006 | |
dc.identifier.issn | 1320-5358 | |
dc.identifier.other | sigarra:79302 | |
dc.identifier.uri | https://hdl.handle.net/10216/1911 | - |
dc.description.abstract | Aim: The uninephrectomised and three-quarter nephrectomised (3/4nx) rats present dopamine-sensitive enhanced natriuresis. This is accompanied in uninephrectomised rats by a reduced jejunal Na+,K+-ATPase activity with recovered sensitivity to inhibition by dopamine. The present study examined the jejunal Na+,K+-ATPase activity and the role of dopamine in 3/4nx animals. Methods: Fourteen days after surgery, the L-amino acid decarboxylase activity (AADC) activity, the enzyme responsible for the synthesis of dopamine, and the Na+,K+-ATPase activity, were determined in jejunal epithelial cells from 3/4nx and Sham rats. In addition, the effect of dopamine (1 mu mol/L) on jejunal Na+,K+-ATPase activity was evaluated in both groups. Results: The 3/4nx rats presented a reduced AADC activity in jejunal epithelial cells (V-max in nmol/mg prot/15 min, 142 +/- 6 vs 190 +/- 10, P < 0.05). In addition, the jejunal Na+,K+-ATPase activity was increased in 3/4nx rats (Pi release in nmol/mg prot/min, 137 +/- 1 vs 122 +/- 2, P < 0.05). However, dopamine was unable to inhibit the Na+,K+-ATPase activity in jejunal epithelial cells from both 3/4nx and Sham animals. Conclusions: In contrast to uninephrectomy, the jejunal Na+,K+-ATPase activity is increased in 3/4nx rats and is not sensitive to inhibition by dopamine. | |
dc.language.iso | eng | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | |
dc.subject | Ciências da Saúde | |
dc.subject | Health sciences | |
dc.title | Jejunal dopamine and Na+,K+-ATPase activity in early chronic renal insufficiency | |
dc.type | Artigo em Revista Científica Internacional | |
dc.contributor.uporto | Faculdade de Medicina Dentária | |
dc.contributor.uporto | Faculdade de Medicina | |
dc.identifier.doi | 10.1111/j.1440-1797.2006.00533.x | |
dc.identifier.authenticus | P-004-NJ2 | |
Appears in Collections: | FMDUP - Artigo em Revista Científica Internacional FMUP - Artigo em Revista Científica Internacional |
This item is licensed under a Creative Commons License