Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/1911
Full metadata record
DC FieldValueLanguage
dc.creatorMaria Maia
dc.creatorPaula Serrão
dc.creatorMaria Manuela Moura
dc.creatorManuel Pestana
dc.date.accessioned2022-09-12T04:56:47Z-
dc.date.available2022-09-12T04:56:47Z-
dc.date.issued2006
dc.identifier.issn1320-5358
dc.identifier.othersigarra:79302
dc.identifier.urihttps://hdl.handle.net/10216/1911-
dc.description.abstractAim: The uninephrectomised and three-quarter nephrectomised (3/4nx) rats present dopamine-sensitive enhanced natriuresis. This is accompanied in uninephrectomised rats by a reduced jejunal Na+,K+-ATPase activity with recovered sensitivity to inhibition by dopamine. The present study examined the jejunal Na+,K+-ATPase activity and the role of dopamine in 3/4nx animals. Methods: Fourteen days after surgery, the L-amino acid decarboxylase activity (AADC) activity, the enzyme responsible for the synthesis of dopamine, and the Na+,K+-ATPase activity, were determined in jejunal epithelial cells from 3/4nx and Sham rats. In addition, the effect of dopamine (1 mu mol/L) on jejunal Na+,K+-ATPase activity was evaluated in both groups. Results: The 3/4nx rats presented a reduced AADC activity in jejunal epithelial cells (V-max in nmol/mg prot/15 min, 142 +/- 6 vs 190 +/- 10, P < 0.05). In addition, the jejunal Na+,K+-ATPase activity was increased in 3/4nx rats (Pi release in nmol/mg prot/min, 137 +/- 1 vs 122 +/- 2, P < 0.05). However, dopamine was unable to inhibit the Na+,K+-ATPase activity in jejunal epithelial cells from both 3/4nx and Sham animals. Conclusions: In contrast to uninephrectomy, the jejunal Na+,K+-ATPase activity is increased in 3/4nx rats and is not sensitive to inhibition by dopamine.
dc.language.isoeng
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.subjectCiências da Saúde
dc.subjectHealth sciences
dc.titleJejunal dopamine and Na+,K+-ATPase activity in early chronic renal insufficiency
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoFaculdade de Medicina Dentária
dc.contributor.uportoFaculdade de Medicina
dc.identifier.doi10.1111/j.1440-1797.2006.00533.x
dc.identifier.authenticusP-004-NJ2
Appears in Collections:FMDUP - Artigo em Revista Científica Internacional
FMUP - Artigo em Revista Científica Internacional

Files in This Item:
File Description SizeFormat 
79302.pdf102.26 kBAdobe PDFThumbnail
View/Open


This item is licensed under a Creative Commons License Creative Commons