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Author(s): Romeu, M.J.L.
Domínguez-Pérez, D.
Almeida, D.
Morais, J.
Campos, A.
Vasconcelos, V.
Mergulhão, F.J.M.
Title: Characterization of planktonic and biofilm cells from two filamentous cyanobacteria using a shotgun proteomic approach
Publisher: Taylor and Francis
Issue Date: 2020
Abstract: Cyanobacteria promote marine biofouling with significant impacts. A qualitative proteomic analysis, by LC-MS/MS, of planktonic and biofilm cells from two cyanobacteria was performed. Biofilms were formed on glass and perspex at two relevant hydrodynamic conditions for marine environments (average shear rates of 4 s−1 and 40 s−1). For both strains and surfaces, biofilm development was higher at 4 s−1. Biofilm development of Nodosilinea sp. LEGE 06145 was substantially higher than Nodosilinea sp. LEGE 06119, but no significant differences were found between surfaces. Overall, 377 and 301 different proteins were identified for Nodosilinea sp. LEGE 06145 and Nodosilinea sp. LEGE 06119. Differences in protein composition were more noticeable in biofilms formed under different hydrodynamic conditions than in those formed on different surfaces. Ribosomal and photosynthetic proteins were identified in most conditions. The characterization performed gives new insights into how shear rate and surface affect the planktonic to biofilm transition, from a structural and proteomics perspective.
Subject: cyanobacterial biofilms
hydrodynamic conditions
Marine biofouling
optical coherence tomography
real-time imaging
shotgun proteomics
Source: Biofouling, 36:6, 631-645
Document Type: Artigo em Revista Científica Internacional
Rights: restrictedAccess
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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