Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/130471
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dc.creatorGirão, M.
dc.creatorRibeiro, I.
dc.creatorRibeiro, T.
dc.creatorAzevedo, I.C.
dc.creatorPereira, F.
dc.creatorUrbatzka, R.
dc.creatorLeão, P.N.
dc.creatorCarvalho, M.F.
dc.date.accessioned2020-12-04T15:17:35Z-
dc.date.available2020-12-04T15:17:35Z-
dc.date.issued2019
dc.identifier.issnISSN 1664-302X
dc.identifier.urihttps://hdl.handle.net/10216/130471-
dc.description.abstractNature is the major reservoir of biologically active molecules. The urgent need of finding novel molecules for pharmaceutical application is prompting the research of underexplored environments, such as marine ecosystems. Here, we investigated cultivable actinobacteria associated with the macroalgae Laminaria ochroleuca and assessed their potential to produce compounds with antimicrobial or anticancer activities. A specimen of L. ochroleuca was collected in a rocky shore in northern Portugal, and fragments of tissues from different parts of the macroalgae (holdfast, stipe, and blades) were surface sterilized and plated in three culture media selective for actinobacteria. A total of 90 actinobacterial strains were isolated, most of which affiliated with the genus Streptomyces. Isolates associated with the genera Isoptericola, Rhodococcus, Nonomuraeae, Nocardiopsis, Microbispora, and Microbacterium were also obtained. Organic extracts from the isolates were tested for their antimicrobial activity using the agar-based disk diffusion method, followed by determination of minimum inhibitory concentration (MIC) values. Forty-five isolates inhibited the growth of Candida albicans and/or Staphylococcus aureus, with MIC values ranging from <0.5 to 1000 μg mL−1. The actinobacterial isolates were also tested for their anticancer potential on two human cancer cell lines. Twenty-eight extracts affected the viability of at least one human cancer cell line (breast carcinoma T-47D and neuroblastoma SH-SY5Y) and non-carcinogenic endothelial cell line (hCMEC/D3). Seven extracts affected the viability of cancer cells only. This study revealed that L. ochroleuca is a rich source of actinobacteria with promising antimicrobial and anticancer activities and suggests that macroalgae may be a valuable source of actinobacteria and, consequently, of new molecules with biotechnological importance.
dc.description.sponsorshipThis work was financed by national funds through Fundação para a Ciência e a Tecnologia (FCT) within the the project IF/00791/2013/CP1197/CT0002, and strategic funding UID/Multi/04423/2019 through funds provided by FCT and ERDF in the framework of the program PT2020. The authors wish to acknowledge grants (IF/00791/2013 to MC and IF/01358/2014 to PL) from the IFCT program supported by FCT, Fundo Social Europeu and Programa Operacional Potencial Humano. RU was supported by a postdoc grant from FCT (SFRH/BPD/112287/2015).
dc.language.isoeng
dc.publisherFrontiers in Microbiology
dc.relation.ispartofFrontiers in Microbiology 10:683
dc.rightsopenAccess
dc.subjectAnticancer
dc.subjectAntimicrobial
dc.subjectBioactivity
dc.subjectEndophytic actinobacteria
dc.subjectKelp
dc.subjectLaminaria ochroleuca
dc.subjectMacroalgae
dc.subjectMarine actinobacteria
dc.titleActinobacteria isolated from laminaria ochroleuca: A source of new bioactive compounds
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoCentro Interdisciplinar de Investigação Marinha e Ambiental
dc.identifier.doihttps://doi.org/10.3389/fmicb.2019.00683
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fmicb.2019.00683/full
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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