Utilize este identificador para referenciar este registo: https://hdl.handle.net/10216/127039
Autor(es): Ferreira, LB
Lima, RT
Bastos, A
Silva, AM
Tavares, C
Pestana, A
Rios, E
Eloy, C
Sobrinho-Simões, M
Gimba, E
Soares, P
Título: OPNa overexpression is associated with matrix calcification in thyroid cancer cell lines
Editor: MDPI
Data de publicação: 2018
Resumo: Osteopontin (OPN) spliced variants (OPN-SV: OPNa, OPNb, and OPNc) are aberrantly expressed in tumors and frequently associated with cancer progression. This holds true for papillary thyroid carcinoma (PTC), which is the most common type of thyroid cancer (TC). PTC often presents with desmoplasia and dystrophic calcification, including psammoma bodies (PB). This work aimed to investigate total OPN (tOPN) and OPN-SV expression and their association with the presence of PB in the PTC classical variants (cPTC), as well as the involvement of OPN-SV in matrix calcification of TC cell lines. We found that cPTC samples presenting PB showed higher OPN expression levels. In TC cell lines, OPNa overexpression promotes higher matrix calcification and collagen synthesis when compared to that of clones overexpressing OPNb or OPNc. In response to OPN knockdown, calcification was inhibited, paralleled with the downregulation of calcification markers. In conclusion, our data evidenced that OPN expression is associated with the presence of PB in cPTC samples. Among the OPN-SV, OPNa is the main contributor to matrix calcification in tested TC cells, providing clues to a better understanding on the biology and ethiopathogenesis of the calcification process in TC cells.
URI: https://hdl.handle.net/10216/127039
Fonte: International Journal of Molecular Sciences, vol.19(10):2990
Informação Relacionada: info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F87887%2F2012/PT
Tipo de Documento: Artigo em Revista Científica Internacional
Condições de Acesso: openAccess
Licença: https://creativecommons.org/licenses/by/4.0/
Aparece nas coleções:I3S - Artigo em Revista Científica Internacional

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
10.3390-ijms19102990.pdf2.87 MBAdobe PDFThumbnail
Ver/Abrir


Este registo está protegido por Licença Creative Commons Creative Commons