Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120729
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dc.creatorPires, L
dc.creatorRocha, D
dc.creatorAmbrosio, L
dc.creatorPêgo, AP
dc.date.accessioned2019-06-25T12:08:37Z-
dc.date.available2019-06-25T12:08:37Z-
dc.date.issued2015
dc.identifier.issn1742-5689
dc.identifier.urihttps://hdl.handle.net/10216/120729-
dc.description.abstractIn tissue engineering, it is well accepted that a scaffold surface has a decisive impact on cell behaviour. Here we focused on microglia-the resident immune cells of the central nervous system (CNS)-and on their response to poly(trimethylene carbonate-co-1-caprolactone) (P(TMC-CL)) fibrous and flat surfaces obtained by electrospinning and solvent cast, respectively. This study aims to provide cues for the design of instructive surfaces that can contribute to the challenging process of CNS regeneration. Cell morphology was evidently affected by the substrate, mirroring the surface main features. Cells cultured on flat substrates presented a round shape, while cells with elongated processes were observed on the electrospun fibres. A higher concentration of the pro-inflammatory cytokine tumour necrosis factor-a was detected in culture media from microglia on fibres. Still, astrogliosis is not exacerbated when astrocytes are cultured in the presence of microgliaconditioned media obtained from cultures in contact with either substrate. Furthermore, a significant percentage of microglia was found to participate in the process of myelin phagocytosis, with the formation of multinucleated giant cells being observed only on films. Altogether, the results presented suggest that microglia in contact with the tested substrates may contribute to the regeneration process, putting forward P(TMC-CL) substrates as supporting matrices for nerve regeneration.
dc.description.sponsorshipThis work was financed by FEDER funds through the Programa Operacional Factores de Competitividade – COMPETE and by Portuguese funds through FCT – Fundação para a Ciência e a Tecnologia in the framework of the project PEst-C/SAU/LA0002/2011. L.P. and D.R. thank FCT for their PhD grants (SFRH/BD/46015/2008 and SFRH/BD/64079/2009).
dc.language.isoeng
dc.publisherRoyal Society
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F46015%2F2008/PT
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F64079%2F2009/PT
dc.relation.ispartofJournal of the Royal Society Interface, vol. 12(103):pii: 20141224
dc.rightsopenAccess
dc.subjectElectrospinning
dc.subjectMicroglia
dc.subjectMultinucleated giant cell
dc.subjectMyelin
dc.subjectNerve tissue engineering
dc.subjectScaffold surface
dc.titleThe role of the surface on microglia function: Implications for central nervous system tissue engineering
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoInstituto de Investigação e Inovação em Saúde
dc.identifier.doi10.1098/rsif.2014.1224
dc.relation.publisherversionhttps://royalsocietypublishing.org/doi/full/10.1098/rsif.2014.1224?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed
Appears in Collections:I3S - Artigo em Revista Científica Internacional

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