Please use this identifier to cite or link to this item: https://hdl.handle.net/10216/120503
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dc.creatorPrieto A.I.
dc.creatorGuzmán-Guillén R.
dc.creatorDíez-Quijada L.
dc.creatorCampos A.
dc.creatorVasconcelos V.
dc.creatorJos Á.
dc.creatorCameán A.M.
dc.date.accessioned2019-05-31T16:16:29Z-
dc.date.available2019-05-31T16:16:29Z-
dc.date.issued2018
dc.identifier.issn20726651
dc.identifier.urihttps://hdl.handle.net/10216/120503-
dc.description.abstractReports on the occurrence of the cyanobacterial toxin cylindrospermopsin (CYN) have increased worldwide because of CYN toxic effects in humans and animals. If contaminated waters are used for plant irrigation, these could represent a possible CYN exposure route for humans. For the first time, a method employing solid phase extraction and quantification by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) of CYN was optimized in vegetables matrices such as lettuce (Lactuca sativa). The validated method showed a linear range, from 5 to 500 ng CYN g−1 of fresh weight (f.w.), and detection and quantitation limits (LOD and LOQ) of 0.22 and 0.42 ng CYN g−1 f.w., respectively. The mean recoveries ranged between 85 and 104%, and the intermediate precision from 12.7 to 14.7%. The method showed to be robust for the three different variables tested. Moreover, it was successfully applied to quantify CYN in edible lettuce leaves exposed to CYN-contaminated water (10 µg L−1), showing that the tolerable daily intake (TDI) in the case of CYN could be exceeded in elderly high consumers. The validated method showed good results in terms of sensitivity, precision, accuracy, and robustness for CYN determination in leaf vegetables such as lettuce. More studies are needed in order to prevent the risks associated with the consumption of CYN-contaminated vegetables. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.sponsorshipAcknowledgments: The authors would like to acknowledge the Ministerio de Economía y Competitividad of Spain (AGL2015-64558-R, MINECO/FEDER, UE) for its financial support and the FCT Project UID/Multi/04423/2013. The authors also gratefully acknowledge the Spanish Ministerio de Economia y Competitividad for the grant FPI (BES-2016-078773) awarded to Leticia Díez-Quijada Jiménez. A. Campos work was supported by a postdoctoral grant (SFRH/BPD/103683/2014) from FCT.
dc.language.isoeng
dc.publisherMDPI
dc.relationinfo:eu-repo/grantAgreement/FCT/5876/147268/PT
dc.relation.ispartofToxins, vol. 10(2):63
dc.rightsopenAccess
dc.subjectcylindrospermopsin
dc.subjectbacterial toxin
dc.subjectcylindrospermopsin
dc.subjecturacil
dc.subjectanalytical parameters
dc.subjectArticle
dc.subjectdrug determination
dc.subjectfreeze drying
dc.subjectlettuce
dc.subjectlimit of detection
dc.subjectlimit of quantitation
dc.subjectlinearity
dc.subjectliquid chromatography-mass spectrometry
dc.subjectmeasurement accuracy
dc.subjectmeasurement precision
dc.subjectnonhuman
dc.subjectplant leaf
dc.subjectrisk assessment
dc.subjectrobustness
dc.subjectruggedness
dc.subjectsensitivity analysis
dc.subjectsolvent extraction
dc.subjectultra performance liquid chromatography
dc.subjectvalidation process
dc.subjectvegetable
dc.subjectanalogs and derivatives
dc.subjectanalysis
dc.subjectchemistry
dc.subjectfood contamination
dc.subjectirrigation (agriculture)
dc.subjectlettuce
dc.subjectliquid chromatography
dc.subjectprocedures
dc.subjecttandem mass spectrometry
dc.subjectvegetable
dc.subjectAgricultural Irrigation
dc.subjectBacterial Toxins
dc.subjectChromatography, Liquid
dc.subjectFood Contamination
dc.subjectLettuce
dc.subjectLimit of Detection
dc.subjectPlant Leaves
dc.subjectTandem Mass Spectrometry
dc.subjectUracil
dc.subjectVegetables
dc.titleValidation of a method for cylindrospermopsin determination in vegetables: Application to real samples such as lettuce (lactuca sativa l.)
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoCIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental
dc.identifier.doi10.3390/toxins10020063
dc.relation.publisherversionhttp://dx.doi.org/10.3390/toxins10020063
Appears in Collections:CIIMAR - Artigo em Revista Científica Internacional

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