Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10216/115899
Autor(es): | Berta N. Estevinho Nuria Samaniego David Talens-Perales Maria José Fabra Amparo López-Rubi Julio Polaina Julia Marín-Navarro |
Título: | Development of enzymatically-active bacterial cellulose membranes through stable immobilization of an engineered beta-galactosidase |
Data de publicação: | 2018 |
Resumo: | Enzymatically-active bacterial cellulose (BC) was prepared by non-covalent immobilization of a hybrid enzyme composed by a beta-galactosidase from Thermotoga maritima (TmLac) and a carbohydrate binding module (CBM2) from Pyrococcus furiosus. TmLac-CBM2 protein was bound to BC, with higher affinity at pH 6.5 than at pH 8.5 and with high specificity compared to the non-engineered enzyme. Both hydrated (HBC) and freeze-dried (DBC) bacterial cellulose showed equivalent enzyme binding efficiencies. Initial reaction rate of HBC-bound enzyme was higher than DBC-bound and both of them were lower than the free enzyme. However, enzyme performance was similar in all three cases for the hydrolysis of 5% lactose to a high extent. Reuse of the immobilized enzyme was limited by the stability of the beta-galactosidase module, whereas the CBM2 module provided stable attachment of the hybrid enzyme to the BC support, after long incubation periods (3 h) at 75 degrees C. |
URI: | https://hdl.handle.net/10216/115899 |
Tipo de Documento: | Artigo em Revista Científica Internacional |
Condições de Acesso: | restrictedAccess |
Aparece nas coleções: | FEUP - Artigo em Revista Científica Internacional |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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287365.pdf Restricted Access | 1.24 MB | Adobe PDF | Request a copy from the Author(s) |
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