Please use this identifier to cite or link to this item:
https://hdl.handle.net/10216/115858
Author(s): | Rui M. Gil da Costa Rita Araújo Joana M. O. Santos Mara Fernandes Tiago Neto Hugo Sousa Joana Ribeiro Margarida M. S. M. Bastos Paula A. Oliveira Diogo Carmo Fátima Casaca Sandra Silva Carlos Lopes Rui Medeiros |
Title: | Regulation of miRNA-146a and miRNA-150 Levels by Celecoxib in Premalignant Lesions of K14-HPV16 Mice |
Issue Date: | 2017 |
Abstract: | Background/Aim: Human papillomavirus type 16 (HPV16) induces various types of cancer in several locations. Microenvironmental microRNAs (miRNAs) such as miRNA-146a and miRNA-150 regulate cancer-associated inflammation and are involved in HPV-induced carcinogenesis. We studied the effects of celecoxib on the expression of these two miRNAs in HPV16-induced lesions. Materials and Methods: Female transgenic (HPV16(+/-)) and wild-type (HPV16(-/-)) mice were administered 75 mg/kg/day celecoxib orally (treatment groups) or placebo (control groups) for four weeks. Skin samples were classified histologically, or used for miRNA analysis by quantitative real-time PCR. Results: HPV16(+/-) mice showed higher miRNA-146a and miRNA-150 expression levels compared to wild-type animals. Celecoxib further increased miRNA-150 (p<0.05) and miRNA-146a levels in treated animals. Celecoxib-treated HPV16(+/-) animals also showed reduced incidence of epidermal dysplasia and reduced inflammation, compared to untreated mice. Conclusion: In this model, celecoxib may be able to regulate tumour-associated inflammation, through mechanisms involving the regulation of miRNA-146a and miRNA-150. |
URI: | https://hdl.handle.net/10216/115858 |
Related Information: | info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e a Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | restrictedAccess |
Appears in Collections: | FEUP - Artigo em Revista Científica Internacional |
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273082.pdf Restricted Access | 2.1 MB | Adobe PDF | Request a copy from the Author(s) |
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