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dc.creatorJoana A. Loureiro
dc.creatorBárbara Gomes
dc.creatorGert Fricker
dc.creatorIsabel Cardoso
dc.creatorCarlos A. Ribeiro
dc.creatorCristiana Gaiteiro
dc.creatorManuel A. N. Coelho
dc.creatorSandra Rocha
dc.creatorMaria do Carmo Pereira
dc.description.abstractDrug delivery systems that can reach brain areas affected by amyloid deposits are still underdeveloped. We propose pegylated liposomes functionalized with two antibodies, the anti-transferrin receptor monoclonal antibody (0X26MAb) and the anti-amyloid beta peptide antibody (19B8MAb), as nanocarriers of drugs for Alzheimer's disease therapy. Two distinct conjugation methods are investigated. In one formulation, the OX26MAb is conjugated to the tip of polyethylene glycol molecules through the maleimide group and the 19B8MAb is bound through the streptavidin biotin complex. In the second system the conjugation reagents are swapped between the antibodies. Fluorescence spectroscopy experiments on porcine brain capillary endothelial cells show that the cellular uptake of the immunoliposomes is substantially more efficient if OX26MAb antibody is conjugated through the streptavidin biotin complex instead of the maleimide group. The ability of the immunoliposomes to cross the blood brain barrier was established by in vivo studies in wild type rats. Our results demonstrate the importance of the conjugation method used to bind the antibody that targets the blood brain barrier to immunoliposomes for efficient drug delivery to the brain.
dc.relationinfo:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/PTDC/QUI-BIQ/118076/2010/Liposomas funcionalizados com anticorpos para a doença de Alzheimer/PTDC/QUI-BIQ/118076/2010
dc.relationinfo:eu-repo/grantAgreement/FCT - Fundação para a Ciência e Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE
dc.relationinfo:eu-repo/grantAgreement/Autoriadade de Gestão do Programa Operacional Regional do Norte/Programas Integrados de IC&DT/NORTE-07-0124-FEDER-000025/(Bio) Chemical Engineering: Multi-Scale Approaches for Sustainable Environment and Health/LEPAE/CEFT - RL2
dc.titleDual ligand immunoliposomes for drug delivery to the brain
dc.typeArtigo em Revista Científica Internacional
dc.contributor.uportoFaculdade de Engenharia
Appears in Collections:FEUP - Artigo em Revista Científica Internacional

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