Utilize este identificador para referenciar este registo:
https://hdl.handle.net/10216/103109
Autor(es): | Sílvia Fontenete Carvalho, D Lourenco, A Guimaraes, N Madureira, P Figueiredo C Azevedo, NF |
Título: | FISHji: New ImageJ macros for the quantification of fluorescence in epifluorescence images |
Data de publicação: | 2016 |
Resumo: | Fluorescence in situ hybridization (FISH) is based on the use of fluorescent staining dyes, however, the signal intensity of the images obtained by microscopy is seldom quantified with accuracy by the researcher. The development of innovative digital image processing programs and tools has been trying to overcome this problem, however, the determination of fluorescent intensity in microscopy images still has issues due to the lack of precision in the results and the complexity of existing software. This work presents FISHji, a set of new ImageJ methods for automated quantification of fluorescence in images obtained by epifluorescence microscopy. To validate the methods, results obtained by FISHji were compared with results obtained by flow cytometry. The mean correlation between FISHji and flow cytometry was high and significant, showing that the imaging methods are able to accurately assess the signal intensity of fluorescence images. FISHji are available for non-commercial use at http://paginas.fe.up.pt/similar to nazevedo/. |
URI: | https://hdl.handle.net/10216/103109 |
Informação Relacionada: | info:eu-repo/grantAgreement/Autoridade de Gestão do Programa Operacional Regional do Norte/Programas Integrados de IC&DT/NORTE-07-0124-FEDER-000025/(Bio) Chemical Engineering: Multi-Scale Approaches for Sustainable Environment and Health/LEPAE/CEFT - RL2 info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e a Tecnologia/Projectos de Investigação Clínica/PIC/IC/82815/2007/Desenvolvimento e aplicação de mímicos de DNA para a rápida identificação de microrganismos patogénicos/DNA mimics info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e a Tecnologia/Projetos Estratégicos/UID/EQU/00511/2013 - POCI-01-0145-FEDER-006939/Laboratório de Engenharia de Processos, Ambiente, Biotecnologia e Energia/LEPABE |
Tipo de Documento: | Artigo em Revista Científica Internacional |
Condições de Acesso: | restrictedAccess |
Aparece nas coleções: | FEUP - Artigo em Revista Científica Internacional FMUP - Artigo em Revista Científica Internacional |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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143174.pdf Restricted Access | Artigo original publicado | 1.93 MB | Adobe PDF | Request a copy from the Author(s) |
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