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https://hdl.handle.net/10216/103079
Author(s): | Ivone Peres Sandra Rocha Joana A. Loureiro Maria do Carmo Pereira Galya Ivanova Manuel Coelho |
Title: | Carbohydrate particles as protein carriers and scaffolds: physico-chemical characterization and collagen stability |
Issue Date: | 2012 |
Abstract: | The preservation of protein properties after entrapping into polymeric matrices and the effects of drying the emulsions still remains uncertain and controversial. Carbohydrate particles were designed and prepared by homogenization of gum arabic and maltodextrin mixture, with collagen hydrolysate (CH) followed by spray-drying. The encapsulation of CH in the carbohydrate matrix was achieved with an efficiency of 85 +/- 2 %. The morphology and the size of the particles, before (40-400 nm) and after spray-drying (<20 mu m), were characterized by scanning electron microscopy and dynamic light scattering. Measurements of the nuclear relaxation times and application of diffusion ordered spectroscopy, obtained through pulsed field gradient NMR experiments, have been performed to determine the structure of the CH-polysaccharide conjugates and to clarify the mechanism of CH immobilization in the polysaccharide matrix. In vitro release profiles in ultrapure water and in cellular medium reveal that the diffusion rate of CH from the polymeric matrix to the dialysis solution decreases in average 30-50 % over time, compared to free CH molecules. In cellular medium at 37 degrees C, the complete release of CH from the particles is achieved only after 24 h, demonstrating a significant decrease in the CH mass transfer process when compared with free CH. The findings of this study outline the ability of gum arabic/maltodextrin matrices to entrap and preserve CH original properties after the spray-drying process and support the potential of the polymeric scaffold for protein delivery and tissue engineering. |
Subject: | Engenharia dos materiais Materials engineering |
Scientific areas: | Ciências da engenharia e tecnologias::Engenharia dos materiais Engineering and technology::Materials engineering |
URI: | https://hdl.handle.net/10216/103079 |
Related Information: | info:eu-repo/grantAgreement/FCT - Fundação para a Ciência e a Tecnologia/Projectos de I&DT em Todos os Domínios Científicos/PTDC/QUI-BIQ/115449/2009/Distibuição de biomoleculas através de nanopartículas inorganica/organicas /BIONANO |
Document Type: | Artigo em Revista Científica Internacional |
Rights: | restrictedAccess |
Appears in Collections: | FEUP - Artigo em Revista Científica Internacional |
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92463.pdf Restricted Access | Artigo original publicado | 617.39 kB | Adobe PDF | Request a copy from the Author(s) |
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